Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO sub(2)

The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical synthesis. In Shewanella oneidensis MR-1...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2016-12, Vol.17 (24), p.2324-2333
Main Authors: Ainsworth, Emma V, Lockwood, Colin WJ, White, Gaye F, Hwang, Ee Taek, Sakai, Tsubasa, Gross, Manuela A, Richardson, David J, Clarke, Thomas A, Jeuken, Lars JC, Reisner, Erwin, Butt, Julea N
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Language:English
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Shewanella oneidensis
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Summary:The transfer of photoenergized electrons from extracellular photosensitizers across a bacterial cell envelope to drive intracellular chemical transformations represents an attractive way to harness nature's catalytic machinery for solar-assisted chemical synthesis. In Shewanella oneidensis MR-1 (MR-1), trans-outer-membrane electron transfer is performed by the extracellular cytochromes MtrC and OmcA acting together with the outer-membrane-spanning porincytochrome complex (MtrAB). Here we demonstrate photoreduction of solutions of MtrC, OmcA, and the MtrCAB complex by soluble photosensitizers: namely, eosinY, fluorescein, proflavine, flavin, and adenine dinucleotide, as well as by riboflavin and flavin mononucleotide, two compounds secreted by MR-1. We show photoreduction of MtrC and OmcA adsorbed on Ru super(II)-dye-sensitized TiO sub(2) nanoparticles and that these protein-coated particles perform photocatalytic reduction of solutions of MtrC, OmcA, and MtrCAB. These findings provide a framework for informed development of strategies for using the outer-membrane-associated cytochromes of MR-1 for solar-driven microbial synthesis in natural and engineered bacteria. Pump priming: Outer-membrane cytochromes provide conduits for electron exchange between bacteria and their environment. Photoreduction of these cytochromes has been achieved. This research paves the way to light-driven microbial synthesis by use of extracellular photosensitizers to harness nature's versatile catalytic machinery.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201600339