Bioluminescence-Sensing Assay for Microbial Growth Recognition

The conventional methods for microbial viability quantification require cultivation and are laborious. There is consequently a widespread need for cultivation-free methods. The adenosine triphosphate (ATP) bioluminescence-sensing assay is considered an extremely effective biosensor; hence ATP is the...

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Bibliographic Details
Published in:Journal of sensors 2016-01, Vol.2016 (2016), p.1-5
Main Authors: Dai, Tianhong, El Tahan, Mahmoud Helmy, Abdel-Kader, Nora S., Eed, Heba Ramadan, Amin, Rehab
Format: Article
Language:English
Subjects:
Adenosine
Adenosine triphosphate
Antibiotics
Assaying
ATP
Bacteria
Bioluminescence
Biosensors
Colonies & territories
Deoxyribonucleic acid
DNA
E coli
Escherichia coli
Feeds
Food
Methods
Microorganisms
Molecular biology
Proteins
Recombinant
Viability
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Summary:The conventional methods for microbial viability quantification require cultivation and are laborious. There is consequently a widespread need for cultivation-free methods. The adenosine triphosphate (ATP) bioluminescence-sensing assay is considered an extremely effective biosensor; hence ATP is the energy currency of all living microbes and can be used as a rapid indicator of microbial viability. We developed an ATP bioluminescence-sensing assay to detect microbial viability. A bioluminescent recombinant E. coli strain was used with luciferase extracted from transformed bacteria. Results showed that there is a direct correlation between the bioluminescence intensity of the ATP bioluminescence-sensing assay and the microbial viability. Bacterial counts from food samples were detected using the developed sensing assay and validated by the traditional plate-counting method. Compared with the plate-counting method, ATP bioluminescence-sensing assay is a more rapid and efficient approach for detecting microbial viability.
ISSN:1687-725X
1687-7268
DOI:10.1155/2016/1492467