BCFA suppresses LPS induced IL-8 mRNA expression in human intestinal epithelial cells

Abstract Branched chain fatty acids (BCFA) are components of common food fats and are major constituents of the normal term human newborn GI tract. Polyunsaturated fatty acids (PUFA) have been suggested to reduce the risk and development of inflammatory bowel diseases (IBD); however, little is known...

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2017-01, Vol.116, p.27-31
Main Authors: Yan, Y, Wang, Z, Greenwald, J, Kothapalli, K.S.D, Park, H.G, Liu, R, Mendralla, E, Lawrence, P, Wang, X, Brenna, J.T
Format: Article
Language:English
Subjects:
Advanced Basic Science
BCFA
Caco-2 Cells
Cell Survival - drug effects
Docosahexaenoic Acids - pharmacology
Eicosapentaenoic Acid - pharmacology
Endocrinology & Metabolism
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - immunology
Fatty Acids - pharmacology
Gene Expression Regulation - drug effects
Humans
IL-8
Inflammation
Interleukin-8 - genetics
Intestines - cytology
Intestines - drug effects
Intestines - immunology
Lipopolysaccharides - adverse effects
NF-kappa B - metabolism
RNA, Messenger - genetics
Signal Transduction - drug effects
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Summary:Abstract Branched chain fatty acids (BCFA) are components of common food fats and are major constituents of the normal term human newborn GI tract. Polyunsaturated fatty acids (PUFA) have been suggested to reduce the risk and development of inflammatory bowel diseases (IBD); however, little is known about the influence of BCFA on inflammation. We investigated the effect of BCFA on interleukin (IL)-8 and NF-κB production in a human intestinal epithelial cell line (Caco-2). Cells were pre-treated with specific BCFA, or DHA, or EPA, and then activated with lipopolysaccharide (LPS). Both anteiso- and iso- BCFA reduce IL-8. Anteiso- BCFA more effectively suppressed IL-8 than iso- BCFA in LPS stimulated Caco-2 cells. However BCFA in general were less effective than DHA or EPA. Activated BCFA-treated cells expressed less of the cell surface Toll-like receptor 4 (TLR-4) compared to controls. These are the first data to show the reduction of pro-inflammatory markers in human cells mediated by BCFA.
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2016.12.001