Mucin-like protein, a saliva component involved in brown planthopper virulence and host adaptation

[Display omitted] •NlMul, a salivary sheath and gel protein, essential for normal sheath formation.•NlMul expression was enhanced when feeding on the resistant rice variety.•NlMul is essential for brown planthopper feeding on the resistant rice plant.•First gene confirmed to be related to planthoppe...

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Bibliographic Details
Published in:Journal of insect physiology 2017-04, Vol.98, p.223-230
Main Authors: Huang, Hai-Jian, Liu, Cheng-Wen, Xu, Hai-Jun, Bao, Yan-Yuan, Zhang, Chuan-Xi
Format: Article
Language:English
Subjects:
Animals
Antibiosis
Feeding Behavior
Hemiptera - genetics
Hemiptera - physiology
Host resistance
Insect Proteins - genetics
Insect Proteins - metabolism
Insect virulence
Mucin-like protein
Mucins - genetics
Mucins - metabolism
Nilaparvata lugens
Oryza - physiology
RNA Interference
RNA, Double-Stranded - genetics
RNA, Double-Stranded - metabolism
Saliva
Saliva - chemistry
Sequence Analysis, DNA
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Summary:[Display omitted] •NlMul, a salivary sheath and gel protein, essential for normal sheath formation.•NlMul expression was enhanced when feeding on the resistant rice variety.•NlMul is essential for brown planthopper feeding on the resistant rice plant.•First gene confirmed to be related to planthopper virulence and host adaptation. The rice brown planthopper (BPH), Nilaparvata lugens, can rapidly adapt to new resistant rice varieties within several generations, rendering its management burdensome. However, the molecular mechanism underlying its adaptability remains unclear. In this study, we investigated the potential role of mucin-like protein (NlMul) in N. lugens virulence and adaptation to host resistance. NlMul is an important glycoprotein that constitutes both gelling and watery saliva, and specifically expressed in the salivary glands at all developmental stages except the egg period. Knocking down the expression of NlMul resulted in the secretion of short and single-branched salivary sheaths. NlMul might help BPH deal with plant resistance, and altered gene expression was observed when BPHs were transferred from a susceptible rice variety to a resistant one. The NlMul-deficient BPHs showed disordered developmental duration and a portion of these insects reared on resistant rice exhibited lethal effects. Our results uncover a saliva-mediated interaction between insect and host plant, and provide useful information in rice breeding and planthopper management.
ISSN:0022-1910
1879-1611
DOI:10.1016/j.jinsphys.2017.01.012