Pseudomonas aeruginosa produces aspirin insensitive eicosanoids and contributes to the eicosanoid profile of polymicrobial biofilms with Candida albicans

Abstract The interaction of clinically relevant microorganisms is the focus of various studies, e.g. the interaction between the pathogenic yeast, Candida albicans , and the bacterium, Pseudomonas aeruginosa . During infection both release arachidonic acid, which they can transform into eicosanoids....

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Bibliographic Details
Published in:Prostaglandins, leukotrienes and essential fatty acids leukotrienes and essential fatty acids, 2017-02, Vol.117, p.36-46
Main Authors: Fourie, Ruan, Ells, Ruan, Kemp, Gabré, Sebolai, Olihile M, Albertyn, Jacobus, Pohl, Carolina H
Format: Article
Language:English
Subjects:
Acetylsalicylic acid
Advanced Basic Science
Arachidonic Acid - metabolism
Aspirin - pharmacology
Biofilm
Biofilms - drug effects
Candida albicans
Candida albicans - physiology
Chromatography, Liquid
Dinoprostone - metabolism
Eicosanoid
Eicosanoids - analysis
Eicosanoids - metabolism
Endocrinology & Metabolism
Masoprocol - pharmacology
Nordihydroguaiaretic acid
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - physiology
Tandem Mass Spectrometry
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Summary:Abstract The interaction of clinically relevant microorganisms is the focus of various studies, e.g. the interaction between the pathogenic yeast, Candida albicans , and the bacterium, Pseudomonas aeruginosa . During infection both release arachidonic acid, which they can transform into eicosanoids. This study evaluated the production of prostaglandin E2 , prostaglandin F2α and 15-hydroxyeicosatetraenoic acid by biofilms of P. aeruginosa and C. albicans. The influence of co-incubation, acetylsalicylic acid and nordihydroguaiaretic acid on biofilm formation and eicosanoid production was evaluated. Acetylsalicylic acid decreased colony forming units of P. aeruginosa , but increased metabolic activity and eicosanoid production of the cells. In contrast to prostaglandin E2 , prostaglandin F2a production by C. albicans was insensitive to acetylsalicylic acid, indicating that different enzymes are responsible for their production in this yeast. Nordihydroguaiaretic acid inhibited biofilm formation by P. aeruginosa , however co-incubation provided protection against this inhibitor. Production of these eicosanoids could affect pathogen-clearance and infection dynamics and this previously uncharacterized facet of interaction could facilitate novel therapeutic intervention against polymicrobial infection.
ISSN:0952-3278
1532-2823
DOI:10.1016/j.plefa.2017.01.008