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Event-specific qualitative and quantitative detection of five genetically modified rice events using a single standard reference molecule
•We developed a PCR detection method for five GM rice events.•A standard plasmid was developed as a calibrator for detecting five GM rice events.•The method can be used to monitor unauthorized GM rice in food and feed.•The method will be specified in the Korean Food Standards Codex. One novel standa...
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Published in: | Food chemistry 2017-07, Vol.226, p.187-192 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | •We developed a PCR detection method for five GM rice events.•A standard plasmid was developed as a calibrator for detecting five GM rice events.•The method can be used to monitor unauthorized GM rice in food and feed.•The method will be specified in the Korean Food Standards Codex.
One novel standard reference plasmid, namely pUC-RICE5, was constructed as a positive control and calibrator for event-specific qualitative and quantitative detection of genetically modified (GM) rice (Bt63, Kemingdao1, Kefeng6, Kefeng8, and LLRice62). pUC-RICE5 contained fragments of a rice-specific endogenous reference gene (sucrose phosphate synthase) as well as the five GM rice events. An existing qualitative PCR assay approach was modified using pUC-RICE5 to create a quantitative method with limits of detection correlating to approximately 1–10 copies of rice haploid genomes. In this quantitative PCR assay, the square regression coefficients ranged from 0.993 to 1.000. The standard deviation and relative standard deviation values for repeatability ranged from 0.02 to 0.22 and 0.10% to 0.67%, respectively. The Ministry of Food and Drug Safety (Korea) validated the method and the results suggest it could be used routinely to identify five GM rice events. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2017.01.065 |