From untargeted LC-QTOF analysis to characterisation of opines in abalone adductor muscle: Theory meets practice

•Opines are important anaerobic end-products of functional and environmental hypoxia in abalone.•Theoretical butylated alanopine was matched to practical abalone findings.•Synthesised opine standards added confidence to the results.•Mass, formula, MS spectra and retention time comparisons confirmed...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-12, Vol.1071, p.44-48
Main Authors: Venter, Leonie, Jansen van Rensburg, Peet J., Loots, Du Toit, Vosloo, Andre, Lindeque, Jeremie Zander
Format: Article
Language:English
Subjects:
Abalone
Alanopine
Mass spectrometry
Opines
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Summary:•Opines are important anaerobic end-products of functional and environmental hypoxia in abalone.•Theoretical butylated alanopine was matched to practical abalone findings.•Synthesised opine standards added confidence to the results.•Mass, formula, MS spectra and retention time comparisons confirmed results.•Abalone alanopine, lysopine, strombine and tauropine were successfully characterised. Abalone have a unique ability to use pyruvate, various amino acids and dehydrogenases, to produce opines as means to prevent the accumulation of NADH during anaerobic conditions. In this study, the theoretical masses, formulae and fragment patterns of butylated opines were used to predict which of these compounds could be found in the abalone adductor muscle using untargeted liquid chromatography quadrupole time-of flight-mass spectrometry. These findings were validated using synthesised opine standards. In essence alanopine, lysopine, strombine and tauropine produced in abalone adductor muscle could be characterised using the highest identification confidence levels.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2017.01.025