Binding, internalisation and degradation of histatin 3 in histatin-resistant derivatives of Candida albicans

The antifungal mechanism of salivary histatin has been studied in Candida albicans and involves binding to a specific receptor, translocation across the membrane and targeting intracellularly. Cell death correlates with non-lytic release of ATP that may function as a cytotoxic mediator extracellular...

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Bibliographic Details
Published in:FEMS microbiology letters 2003-03, Vol.220 (2), p.247-253
Main Authors: Fitzgerald, Deirdre H, Coleman, David C, O’Connell, Brian C
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Antifungal Agents - metabolism
Antifungal mechanism
Biodegradation, Environmental
Biological and medical sciences
Candida albicans
Candida albicans - drug effects
Candida albicans - metabolism
Drug Resistance, Fungal
Fundamental and applied biological sciences. Psychology
Histatin
Histatin resistance
Histatins
Microbial Sensitivity Tests
Microbiology
Miscellaneous
Mutation
Mycology
Oxygen - metabolism
Protein Binding
Protein Transport
Proteins - metabolism
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Summary:The antifungal mechanism of salivary histatin has been studied in Candida albicans and involves binding to a specific receptor, translocation across the membrane and targeting intracellularly. Cell death correlates with non-lytic release of ATP that may function as a cytotoxic mediator extracellularly. By sequential exposure to increasing concentrations of histatin 3, we generated histatin-resistant derivatives of C. albicans strain CA132A that show five-fold less killing at physiological concentrations of histatin 3. Protection against histatin killing in histatin-resistant derivatives is not due to alterations in binding, internalisation or degradation of histatin or efflux of ATP. These results indicate that protective mechanisms activated by exposure to histatin 3 may involve unidentified pathways downstream of binding and internalisation events.
ISSN:0378-1097
1574-6968
DOI:10.1016/S0378-1097(03)00121-6