Use of Membrane Potential to Achieve Transmembrane Modification with an Artificial Receptor

We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that wa...

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Bibliographic Details
Published in:Bioconjugate chemistry 2017-02, Vol.28 (2), p.296-301
Main Authors: Hatanaka, Wataru, Kawaguchi, Miki, Sun, Xizheng, Nagao, Yusuke, Ohshima, Hiroyuki, Hashida, Mitsuru, Higuchi, Yuriko, Kishimura, Akihiro, Katayama, Yoshiki, Mori, Takeshi
Format: Article
Language:English
Subjects:
Cell Membrane - metabolism
Cells
Humans
Hydrophobic and Hydrophilic Interactions
Intracellular Space - metabolism
K562 Cells
Membrane Potentials
Membranes
Peptides
Polyethylene Glycols - chemistry
Proteins
Receptors, Artificial - chemistry
Receptors, Artificial - metabolism
Solubility
Streptavidin - metabolism
Water - chemistry
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Summary:We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.
ISSN:1043-1802
1520-4812
DOI:10.1021/acs.bioconjchem.6b00449