Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates

• Published in: Acta biomaterialia 2014-03, Vol.10 (3), p.1354-1359
• Main Authors: Seras-Franzoso, Joaquin, Peebo, Karl, García-Fruitós, Elena, Vázquez, Esther, Rinas, Ursula, Villaverde, Antonio
• Format: Article
• Language: English
• Subjects: Animals; Bacteria; Biological activity; Biotechnology; Cell Culture Techniques; Delivery systems; Drug delivery; Drug Delivery Systems; Escherichia coli; Escherichia coli - metabolism; Fibroblast Growth Factor 2 - pharmacology; Fibroblasts; HeLa Cells; Hep G2 Cells; Humans; Inclusion bodies; Inclusion Bodies - chemistry; Inclusion Bodies - drug effects; Inclusions; Mice; Nanomedicine; NIH 3T3 Cells; PC12 Cells; Protein release; Proteins; Rats; Surface functionalization; Topography
• ISSN: 1742-7061; 1878-7568
• DOI: 10.1016/j.actbio.2013.12.021

Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37°C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25°C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.