Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM)...

Full description

Saved in:
Bibliographic Details
Published in:Molecular neurobiology 2018-03, Vol.55 (3), p.2285-2300
Main Authors: Baez-Jurado, Eliana, Vega, Gina Guio, Aliev, Gjumrakch, Tarasov, Vadim V., Esquinas, Paula, Echeverria, Valentina, Barreto, George E.
Format: Article
Language:English
Subjects:
Adipose tissue
Adult
Astrocytes - drug effects
Astrocytes - physiology
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Line
Cells, Cultured
Central nervous system
Culture Media, Conditioned - pharmacology
DNA fragmentation
Humans
Membrane potential
Mesenchymal stem cells
Mesenchymal Stem Cells - physiology
Mesenchyme
Mitochondria
Neurobiology
Neuroglobin
Neuroglobin - antagonists & inhibitors
Neuroglobin - physiology
Neurological diseases
Neurology
Neuroprotective Agents - administration & dosage
Neurosciences
Oxidative Stress - drug effects
Oxidative Stress - physiology
Paracrine signalling
Physical Stimulation - adverse effects
Reactive oxygen species
Stem cells
Traumatic brain injury
Wounds
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.
ISSN:0893-7648
1559-1182
DOI:10.1007/s12035-017-0481-y