In Vitro Antifungal Susceptibility of Neoscytalidium dimidiatum Clinical Isolates from Malaysia

Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte in...

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Bibliographic Details
Published in:Mycopathologia (1975) 2017-04, Vol.182 (3-4), p.305-313
Main Authors: James, Jasper Elvin, Santhanam, Jacinta, Lee, Mei Chen, Wong, Choon Xian, Sabaratnam, Parameswari, Yusoff, Hamidah, Tzar, Mohd Nizam, Razak, Mohd Fuat Abdul
Format: Article
Language:English
Subjects:
Antifungal agents
Antifungal Agents - pharmacology
Antiparasitic agents
Ascomycota - drug effects
Ascomycota - isolation & purification
Biomedical and Life Sciences
Dermatomycoses - microbiology
Eukaryotic Microbiology
Foot diseases
Genetic testing
Health aspects
Humans
Infection
Infections
Laboratories
Life Sciences
Malaysia
Medical Microbiology
Medical research
Microbial Ecology
Microbial Sensitivity Tests
Microbiology
Morphology
Plant Sciences
Skin
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Summary:Neoscytalidium dimidiatum is an opportunistic fungus causing cutaneous infections mostly, which are difficult to treat due to antifungal resistance. In Malaysia, N. dimidiatum is associated with skin and nail infections, especially in the elderly. These infections may be mistaken for dermatophyte infections due to similar clinical appearance. In this study, Neoscytalidium isolates from cutaneous specimens, identified using morphological and molecular methods (28 Neoscytalidium dimidiatum and 1 Neoscytalidium sp.), were evaluated for susceptibility towards antifungal agents using the CLSI broth microdilution (M38-A2) and Etest methods. Amphotericin B, voriconazole, miconazole and clotrimazole showed high in vitro activity against all isolates with MIC ranging from 0.0313 to 1 µg/mL. Susceptibility towards fluconazole and itraconazole was noted in up to 10% of isolates, while ketoconazole was inactive against all isolates. Clinical breakpoints for antifungal drugs are not yet available for most filamentous fungi, including Neoscytalidium species. However, the results indicate that clinical isolates of N. dimidiatum in Malaysia were sensitive towards miconazole, clotrimazole, voriconazole and amphotericin B, in vitro.
ISSN:0301-486X
1573-0832
DOI:10.1007/s11046-016-0085-5