Quantitative proteomic analysis of gingival crevicular fluids from deciduous and permanent teeth

Aim Gingivitis commonly progresses to periodontitis in permanent dentition but rarely in deciduous teeth. Little is known about the biochemical differences between gingiva of deciduous and permanent teeth. Here, we compared the protein profiles of gingival crevicular fluids (GCF) from the gingiva of...

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Published in:Journal of clinical periodontology 2017-04, Vol.44 (4), p.353-362
Main Authors: Moriya, Yumi, Obama, Takashi, Aiuchi, Toshihiro, Sugiyama, Tomomi, Endo, Yumiko, Koide, Yoko, Noguchi, Emiko, Ishizuka, Motonori, Inoue, Mitsuko, Itabe, Hiroyuki, Yamamoto, Matsuo
Format: Article
Language:English
Subjects:
Child
deciduous dentition
Dentistry
Dentition, Mixed
Female
gingival crevicular fluid
Gingival Crevicular Fluid - chemistry
Gum disease
Humans
Male
neutrophil function
permanent dentition
Proteomics
quantitative proteomics
Teeth
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Summary:Aim Gingivitis commonly progresses to periodontitis in permanent dentition but rarely in deciduous teeth. Little is known about the biochemical differences between gingiva of deciduous and permanent teeth. Here, we compared the protein profiles of gingival crevicular fluids (GCF) from the gingiva of deciduous and permanent teeth. Materials and Methods Forty children with mixed dentition (Hellman's dental age IIIA) were selected and GCF samples were collected from deciduous cuspids and central incisors in the maxilla. Pairs of GCF samples were labelled using isobaric tags to permit quantitative comparison of protein abundance in the samples using liquid chromatography‐electron spray ionization‐tandem mass spectrometry. Results Sixty‐two proteins were upregulated in deciduous teeth GCF and 54 in permanent teeth GCF. In particular, neutrophil‐derived proteins, including myeloperoxidase and lactoferrin, were repeatedly higher in deciduous teeth GCF than in permanent teeth GCF. These differences were verified using ELISA (p 
ISSN:0303-6979
1600-051X
DOI:10.1111/jcpe.12696