Human Skin 3D Bioprinting Using Scaffold‐Free Approach

Organ in vitro synthesis is one of the last bottlenecks between tissue engineering and transplantation of synthetic organs. Bioprinting has proven its capacity to produce 3D objects composed of living cells but highly organized tissues such as full thickness skin (dermis + epidermis) are rarely atta...

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Bibliographic Details
Published in:Advanced healthcare materials 2017-02, Vol.6 (4), p.np-n/a
Main Authors: Pourchet, Léa J., Thepot, Amélie, Albouy, Marion, Courtial, Edwin J., Boher, Aurélie, Blum, Loïc J., Marquette, Christophe A.
Format: Article
Language:English
Subjects:
3D printing
Adults
Animals
Bioengineering
biomaterials
bioprinting
dermis
Dermis - cytology
Dermis - metabolism
Ear
Electronics
Epidermis - cytology
Epidermis - metabolism
human skin
Humans
Mice
NIH 3T3 Cells
Organs
Printing, Three-Dimensional
Skin (anatomy)
skin equivalent
Three dimensional printing
Tissue engineering
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Summary:Organ in vitro synthesis is one of the last bottlenecks between tissue engineering and transplantation of synthetic organs. Bioprinting has proven its capacity to produce 3D objects composed of living cells but highly organized tissues such as full thickness skin (dermis + epidermis) are rarely attained. The focus of the present study is to demonstrate the capability of a newly developed ink formulation and the use of an open source printer, for the production of a really complete skin model. Proofs are given through immunostaining and electronic microscopy that the bioprinted skin presents all characteristics of human skin, both at the molecular and macromolecular level. Finally, the printability of large skin objects is demonstrated with the printing of an adult‐size ear. Full thickness human skin is printed using an open source 3D bioprinter and a specially developed bio‐ink. The produced skin presents all relevant extracellular matrix proteins and dermal–epidermal junction. Large and complex tissues such as adult human ear are also achieved.
ISSN:2192-2640
2192-2659
DOI:10.1002/adhm.201601101