Prospecting fungal parasites of the potato cyst nematode Globodera pallida using a rapid screening technique

Seven filamentous fungal species were isolated from individual eggs of Globodera pallida cysts collected from infested fields in Shelley Idaho, USA and identified as Chaetomium globosum, Fusarium oxysporum, Fusarium solani, Fusarium tricinctum, Microdochium bolleyi, Purpureocillium lilacinum, and Pl...

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Bibliographic Details
Published in:Journal of basic microbiology 2017-05, Vol.57 (5), p.386-392
Main Authors: Kooliyottil, Rinu, Dandurand, Louise‐Marie, Knudsen, Guy R.
Format: Article
Language:English
Subjects:
Animals
biocontrol agent
fungal antagonist
Fungi - classification
Fungi - genetics
Fungi - isolation & purification
Globodera pallida
nematophagous fungi
Organic Chemicals
Pest Control, Biological - methods
Plant Diseases - microbiology
Plant Diseases - parasitology
Plant Roots - microbiology
Plant Roots - parasitology
potato cyst nematode
Rhizosphere
Soil - parasitology
Soil Microbiology
Solanum tuberosum - microbiology
Solanum tuberosum - parasitology
Tylenchoidea - microbiology
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Summary:Seven filamentous fungal species were isolated from individual eggs of Globodera pallida cysts collected from infested fields in Shelley Idaho, USA and identified as Chaetomium globosum, Fusarium oxysporum, Fusarium solani, Fusarium tricinctum, Microdochium bolleyi, Purpureocillium lilacinum, and Plectosphaerella cucumerina. Their ability to reduce infection by G. pallida in planta were assessed in simple, reproducible micro‐rhizosphere chambers (micro‐ROCs). All fungi reduced G. pallida infection in potato, but greatest reduction was observed with C. globosum at an average reduction of 76%. Further non‐destructive methods were developed to rapidly assess biological control potential of putative fungal strains by staining the infectious second stage juveniles of G. pallida with the live fluorescent stain PKH26. In comparisons between the standard, invasive acid fuchsin method and use of the live stain PKH26, no significant difference in infection level of G. pallida was observed whether roots were stained with PKH26 or acid fuchsin. For both methods, a similar reduction (77% for acid fuchsin, and 78% for PKH26 stain) in invasion of infectious stage of G. pallida was observed when potato plants were inoculated with C. globosum compared to non‐inoculated potato.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201600683