The alternative life of RNA—sequencing meets single molecule approaches

The central dogma of RNA processing has started to totter. Single genes produce a variety of mRNA isoforms by mRNA modification, alternative polyadenylation (APA), and splicing. Different isoforms, even those that code for the identical protein, may differ in function or spatiotemporal expression. O...

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Bibliographic Details
Published in:FEBS letters 2017-06, Vol.591 (11), p.1455-1470
Main Authors: Fernández‐Moya, Sandra M., Ehses, Janina, Kiebler, Michael A.
Format: Article
Language:English
Subjects:
3' Untranslated Regions - genetics
alternative 3′‐UTR
Humans
local translation
Models, Biological
Polyadenylation - genetics
Polyadenylation - physiology
ribonucleoprotein particles
RNA Splicing - genetics
RNA Splicing - physiology
RNA, Messenger - genetics
Sequence Analysis, RNA - methods
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Summary:The central dogma of RNA processing has started to totter. Single genes produce a variety of mRNA isoforms by mRNA modification, alternative polyadenylation (APA), and splicing. Different isoforms, even those that code for the identical protein, may differ in function or spatiotemporal expression. One option of how this can be achieved is by the selective recruitment of trans‐acting factors to the 3′‐untranslated region of a given isoform. Recent innovations in high‐throughput RNA‐sequencing methods allow deep insight into global RNA regulation, whereas novel imaging‐based technologies enable researchers to explore single RNA molecules during different stages of development, in different tissues and different compartments of the cell. Resolving the dynamic function of ribonucleoprotein particles in splicing, APA, or RNA modification will enable us to understand their contribution to pathological conditions.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.12639