Induced hypothermia is associated with reduced circulating subunits of mitochondrial DNA in cardiac arrest patients

• Published in: Mitochondrial DNA. Part A. DNA mapping, sequencing, and analysis sequencing, and analysis, 2018-05, Vol.29 (4), p.525-528
• Main Authors: Aslami, Hamid, Beurskens, Charlotte J P, Tuip, Anita M, Horn, Janneke, Juffermans, Nicole P
• Format: Article
• Language: English
• Subjects: Body Temperature; Case-Control Studies; DNA, Mitochondrial - blood; DNA, Mitochondrial - genetics; Female; Humans; Hypothermia, Induced; Male; Middle Aged; Out-of-Hospital Cardiac Arrest - blood; Out-of-Hospital Cardiac Arrest - genetics; Out-of-Hospital Cardiac Arrest - therapy; Prognosis
• ISSN: 2470-1394; 2470-1408
• DOI: 10.1080/24701394.2017.1315568

Induced hypothermia may protect from ischemia reperfusion injury. The mechanism of protection is not fully understood and may include an effect on mitochondria. Here we describe the effect of hypothermia on circulating mitochondrial (mt) DNA in a substudy of a multicenter randomized trial (the Target Temperature Management trial). Circulating levels of mtDNA were elevated in patients with cardiac arrest at all-time points compared to healthy controls. After 24 h of temperature management, patients kept at 33 °C had significantly lower levels of COX3, NADH1 and NADH2 compared to baseline, in contrast to those kept at 36 °C. After regain of temperature, cytochrome - B was significantly reduced in patients kept at 33 °C with cardiac arrest. Cardiac arrest results in circulating mtDNA levels, which reduced during a temperature management protocol in patients with a target temperature of 33 °C.