Molecular cloning and characterization of the gene encoding osmotin protein in Petunia hybrida

A cDNA clone encoding osmotin, PhOSM, was isolated from a cDNA library constructed from petal protoplast cultures of Petunia hybrida. PhOSM cDNA was composed of an open reading frame corresponding to protein of 246 amino acids and had a calculated molecular weight of 26.7 kDa. Database comparisons o...

Full description

Saved in:
Bibliographic Details
Published in:Plant science (Limerick) 2002-05, Vol.162 (5), p.745-752
Main Authors: Kim, Hoyeun, Mun, Jeong-Hwan, Byun, Bo Hyun, Hwang, Hyun-Ju, Kwon, Young Myung, Kim, Sang-Gu
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
Cell physiology
Classical and quantitative genetics. Population genetics. Molecular genetics
Fundamental and applied biological sciences. Psychology
Gene expression
Generalities. Genetics. Plant material
Genes. Genome
Genetics and breeding of economic plants
Jasmonic acid
Molecular and cellular biology
Molecular genetics
Pathogen
Petunia petal protoplast culture
Signal transduction
Wounding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A cDNA clone encoding osmotin, PhOSM, was isolated from a cDNA library constructed from petal protoplast cultures of Petunia hybrida. PhOSM cDNA was composed of an open reading frame corresponding to protein of 246 amino acids and had a calculated molecular weight of 26.7 kDa. Database comparisons of the PhOSM protein sequences revealed high identity (89%) with the tobacco osmotin and tobacco OLP (Osmotin-Like-Protein). The increased accumulation of PhOSM mRNA in petal protoplast cultures was completely inhibited by treatment with Dhp (3,4-dehydro- l-proline), which is a inhibitor of peptydyl proline hydroxylation in cell wall regeneration. RNA blot analyses revealed that PhOSM was expressed primarily in roots and slightly in the pistil, 3 days after flowering. PhOSM expression was strongly induced in leaves that were exposed to Penicillium funiculosum, Erwinia stewartii and Pseudomonas syringae but not to Aspergillus nidulans. Upon wounding, PhOSM transcripts were induced in the directly damaged leaf but not systemically. Moreover, PhOSM transcript levels increased in response to octadecanoid pathway intermediates and treatments with aspirin and salicylic acid. Our results indicate that PhOSM is developmentally regulated as well as involved in wound-stress signal transduction.
ISSN:0168-9452
1873-2259
DOI:10.1016/S0168-9452(02)00016-X