Determination of reference intervals of acid‐base parameters in clinically healthy dogs

• Published in: Journal of veterinary emergency and critical care (San Antonio, Tex. : 2000) Tex. : 2000), 2017-05, Vol.27 (3), p.325-332
• Main Authors: Vanova‐Uhrikova, Ivana, Rauserova‐Lexmaulova, Leona, Rehakova, Kristina, Scheer, Peter, Doubek, Jaroslav
• Format: Article
• Language: English
• Subjects: Acid-Base Equilibrium; Animals; anion gap; Bicarbonates - blood; Blood Gas Analysis - veterinary; canine; Chlorides - blood; Dogs - blood; Female; Lactic Acid - blood; Male; Potassium - blood; Prospective Studies; Reference Values; strong ion difference; strong ion gap; unmeasured anions
• ISSN: 1479-3261; 1476-4431
• DOI: 10.1111/vec.12589

Objective To establish reference intervals for traditionally‐ and Stewart's approach‐determined acid‐base parameters in a population of clinically healthy dogs. Design Prospective study (June 2011–September 2012). Setting Veterinary teaching hospital. Animals Two hundred twenty‐four client‐owned, clinically healthy dogs. Interventions Blood was collected from the jugular vein and the dorsal pedal artery. Measurements and Main Results In the whole blood samples, pH, PCO2, and PO2 were measured and HCO3−, standard and total bicarbonate, base excess, oxygen content, and alveolar–arterial oxygen differences were calculated. Plasma sodium, potassium, chloride, calcium, albumin, and lactate concentrations were measured and the following parameters were calculated separately for venous and arterial samples: anion gap, anion gap corrected for albumin and phosphate, sodium chloride difference and ratio, chloride gap, strong ion difference, strong ion gap, and unmeasured anions. Conclusion Reference intervals for traditionally‐ and Stewart's approach‐determined acid‐base parameters were established for venous and arterial blood in dogs.