Application of recombinant Pediococcus acidilactici BD16 (fcs+/ech+) for bioconversion of agrowaste to vanillin

• Published in: Applied microbiology and biotechnology 2017-07, Vol.101 (14), p.5615-5626
• Main Authors: Chakraborty, Debkumar, Selvam, Ammaiyappan, Kaur, Baljinder, Wong, Jonathan Woon Chung, Karthikeyan, Obulisamy Parthiba
• Format: Article
• Language: English
• Subjects: Agricultural wastes; Agriculture; Bacteria; Benzaldehydes - metabolism; Bioconversion; Biomass; Biomedical and Life Sciences; Biosynthesis; Biotechnological Products and Process Engineering; Biotechnology; Biotechnology - economics; Biotechnology - methods; Biotransformation; Chemical properties; Enoyl-CoA Hydratase - genetics; Ferulic acid; Genetic recombination; Genetic transformation; Industrial Microbiology - economics; Industrial Microbiology - methods; Industrial production; Lactic acid; Lactic acid bacteria; Lactobacillus; Life Sciences; Metabolic Engineering - economics; Metabolic Engineering - methods; Metabolic Networks and Pathways; Metabolism; Microbial Genetics and Genomics; Microbiology; Oryza - chemistry; Pediococcus acidilactici; Pediococcus acidilactici - genetics; Pediococcus acidilactici - metabolism; Phenolic compounds; Phenols; Phenols - chemistry; Phenols - metabolism; Production processes; Rice bran; Vanillin
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-017-8283-8

Biotechnological production of vanillin is gaining momentum as the natural synthesis of vanillin that is very expensive. Ferulic acid (FA), a costly compound, is used as the substrate to produce vanillin biotechnologically and the making process is still expensive. Therefore, this study investigated the practical use of an agrobiomass waste, rice bran, and provides the first evidence of a cost-effective production of vanillin within 24 h of incubation using recombinant Pediococcus acidilactici BD16 ( fcs + / ech + ). Introduction of two genes encoding feruloyl CoA synthetase and enoyl CoA hydratase into the native strain increased vanillin yield to 4.01 g L −1 . Bioconversion was monitored through the transformation of phenolic compounds. A hypothetical metabolic pathway of rice bran during the vanillin bioconversion was proposed with the inserted pathway from ferulic acid to vanillin and compared with that of other metabolic engineered strains. These results could be a gateway of using recombinant lactic acid bacteria for industrial production of vanillin from agricultural waste.