The calorimetric properties of liposomes determine the morphology of dried droplets

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2017-07, Vol.155, p.215-222
• Main Authors: González-Gutiérrez, Jorge, Pérez-Isidoro, Rosendo, Pérez-Camacho, M.I., Ruiz-Suárez, J.C.
• Format: Article
• Language: English
• Subjects: 1,2-Dipalmitoylphosphatidylcholine - chemistry; Calorimetry; Droplets; Evaporation; Hydrophobic and Hydrophilic Interactions; Lipid Droplets - chemistry; Lipid Droplets - ultrastructure; Liposome; Liposomes - chemistry; Liposomes - ultrastructure; Particle Size; Phase Transition; Phosphatidylglycerols - chemistry; Surface Properties; Temperature
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2017.04.022

[Display omitted] •The calorimetry of suspended vesicles is important in the evaporation of a droplet.•The diameters of dried drops correlate with the transition of lipid vesicles.•Patterns are sensitive to hydrophobic molecules within the suspended vesicles. The evaporation of liquid droplets deposited on a substrate is a very complex phenomenon. Driven by capillary and Marangoni flows, particle–particle and particle–substrate interactions, the deposits they leave are vestiges of such complexity. We study the formation of patterns during the evaporation of liposome suspension droplets deposited on a hydrophobic substrate at different temperatures. We observed that as we change the temperature of the substrate, a morphological phase transition occurs at a given temperature Tm. This temperature corresponds to the gel-fluid lipid melting transition of the liposome suspension. Optical microscopy and atomic force microscopy are used to study the morphology of the patterns. Based on the radial density profiles we found that all structures can be classified into two groups: patterns composed by nearly uniform deposition (below Tm) and prominent structures containing randomly distributed voids (above Tm).