Metabolic engineering of Synechocystis sp. PCC 6803 for enhanced ethanol production based on flux balance analysis

Synechocystis sp. PCC 6803 is an attractive host for bio-ethanol production due to its ability to directly convert atmospheric carbon dioxide into ethanol using photosystems. To enhance ethanol production in Synechocystis sp. PCC 6803, metabolic engineering was performed based on in silico simulatio...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2017-05, Vol.40 (5), p.791-796
Main Authors: Yoshikawa, Katsunori, Toya, Yoshihiro, Shimizu, Hiroshi
Format: Article
Language:English
Subjects:
Ammonium
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Biofuels
Biotechnology
Carbon dioxide
Chemistry
Chemistry and Materials Science
Dehydrogenase
Environmental Engineering/Biotechnology
Ethanol
Ethanol - metabolism
Food Science
Gene Knockdown Techniques
Genome, Bacterial
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Metabolic Engineering - methods
Metabolism
Models, Biological
NADPH Dehydrogenase - genetics
NADPH Dehydrogenase - metabolism
Oxidation
Research Paper
Synechocystis
Synechocystis - genetics
Synechocystis - metabolism
Tissue engineering
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Summary:Synechocystis sp. PCC 6803 is an attractive host for bio-ethanol production due to its ability to directly convert atmospheric carbon dioxide into ethanol using photosystems. To enhance ethanol production in Synechocystis sp. PCC 6803, metabolic engineering was performed based on in silico simulations, using the genome-scale metabolic model. Comprehensive reaction knockout simulations by flux balance analysis predicted that the knockout of NAD(P)H dehydrogenase enhanced ethanol production under photoautotrophic conditions, where ammonium is the nitrogen source. This deletion inhibits the re-oxidation of NAD(P)H, which is generated by ferredoxin-NADP + reductase and imposes re-oxidation in the ethanol synthesis pathway. The effect of deleting the ndhF1 gene, which encodes NADH dehydrogenase subunit 5, on ethanol production was experimentally evaluated using ethanol-producing strains of Synechocystis sp. PCC 6803. The ethanol titer of the ethanol-producing ∆ ndhF1 strain increased by 145%, compared with that of the control strain.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-017-1744-8