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Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease
This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD). We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography...
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| Published in: | Archives of biochemistry and biophysics 2017-06, Vol.623-624, p.64-75 |
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| container_end_page | 75 |
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| container_title | Archives of biochemistry and biophysics |
| container_volume | 623-624 |
| creator | Horas H Nababan, Saut Nishiumi, Shin Kawano, Yuki Kobayashi, Takashi Yoshida, Masaru Azuma, Takeshi |
| description | This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD).
We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography/mass spectrometry (LC/MS). An in vitro experiment was performed using HepG2 cells stimulated with recombinant human TNFα or IL1β. The expression of steatosis-, inflammation-, and fibrosis-related genes were analyzed. Plasma samples from NAFLD patients were also analyzed by LC/MS.
The CDAHFD-fed db/db mice with hepatic steatosis, inflammation, mild fibrosis, obesity, and hypercholesterolemia displayed significantly higher hepatic and plasma levels of free adrenic acid (p |
| doi_str_mv | 10.1016/j.abb.2017.04.009 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1893969959</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003986117301479</els_id><sourcerecordid>1893969959</sourcerecordid><originalsourceid>FETCH-LOGICAL-c481t-dd20d4367253142921366c29285ddd1506c1b76b107c3bc057a5b9020a1bbf303</originalsourceid><addsrcrecordid>eNp9kE1LxDAQhoMo7vrxA7xIj15aZ5o0bfAk4hcoXvQc8lU2S5tq0hX892ZZ9ehpYOZ5X5iHkDOECgH55bpSWlc1YFsBqwDEHlkiCF4C7dg-WQIALUXHcUGOUloDIDJeH5JF3bGGcwZL8nxtowveFMp4W6hUqFD40A9qHNXsp1C4sFLBuJi3RZhCqQYzraYhJ3o1z1_F4D_z0frkVHIn5KBXQ3KnP_OYvN3dvt48lE8v948310-lYR3OpbU1WEZ5WzcUWS1qpJybPLvGWosNcIO65RqhNVQbaFrVaAE1KNS6p0CPycWu9z1OHxuXZjn6ZNwwqOCmTZLYCSq4EI3IKO5QE6eUouvle_Sjil8SQW4tyrXMFuXWogQms8WcOf-p3-jR2b_Er7YMXO0Al5_89C7KZLzLmqyPzszSTv6f-m86noCy</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1893969959</pqid></control><display><type>article</type><title>Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Horas H Nababan, Saut ; Nishiumi, Shin ; Kawano, Yuki ; Kobayashi, Takashi ; Yoshida, Masaru ; Azuma, Takeshi</creator><creatorcontrib>Horas H Nababan, Saut ; Nishiumi, Shin ; Kawano, Yuki ; Kobayashi, Takashi ; Yoshida, Masaru ; Azuma, Takeshi</creatorcontrib><description>This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD).
We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography/mass spectrometry (LC/MS). An in vitro experiment was performed using HepG2 cells stimulated with recombinant human TNFα or IL1β. The expression of steatosis-, inflammation-, and fibrosis-related genes were analyzed. Plasma samples from NAFLD patients were also analyzed by LC/MS.
The CDAHFD-fed db/db mice with hepatic steatosis, inflammation, mild fibrosis, obesity, and hypercholesterolemia displayed significantly higher hepatic and plasma levels of free adrenic acid (p < 0.05). The accumulated adrenic acid in the CDAHFD-fed db/db mice was associated with increased expression of ELOVL2 and 5, and the suppression of the acyl-CoA oxidase 1 gene during peroxisomal β-oxidation. The pretreatment of HepG2 cells with adrenic acid enhanced their cytokine-induced cytokines and chemokines mRNA expression. In NAFLD patients, the group with the highest ALT levels exhibited higher plasma adrenic acid concentrations than the other ALT groups (p-value for trend <0.001).
Data obtained demonstrated that adrenic acid accumulation contributes to disease progression in NAFLD.
•Plasma and hepatic levels of free adrenic acid were markedly increased in a mouse model of steatohepatitis.•Adrenic acid supplementation enhanced cytokine and chemokine gene expression in hepatocytes.•High ALT levels were associated with higher plasma adrenic acid levels in NAFLD patients.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/j.abb.2017.04.009</identifier><identifier>PMID: 28456640</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adrenic acid ; Adult ; Alanine aminotransferase ; Animals ; Chemokine ; Cytokines - analysis ; Cytokines - immunology ; Disease Models, Animal ; Disease Progression ; Fatty Acids, Unsaturated - analysis ; Fatty Acids, Unsaturated - blood ; Fatty Acids, Unsaturated - immunology ; Female ; Gene Expression Regulation ; Hep G2 Cells ; Humans ; Inflammation - blood ; Inflammation - genetics ; Inflammation - immunology ; Inflammation - pathology ; Inflammation Mediators - analysis ; Inflammation Mediators - blood ; Inflammation Mediators - immunology ; Lipids - analysis ; Lipids - blood ; Lipids - immunology ; Liquid chromatography/mass-spectrometry ; Liver - immunology ; Liver - pathology ; Male ; Mice ; Mice, Inbred C57BL ; Middle Aged ; Non-alcoholic fatty liver disease ; Non-alcoholic Fatty Liver Disease - blood ; Non-alcoholic Fatty Liver Disease - genetics ; Non-alcoholic Fatty Liver Disease - immunology ; Non-alcoholic Fatty Liver Disease - pathology ; Oxidative Stress</subject><ispartof>Archives of biochemistry and biophysics, 2017-06, Vol.623-624, p.64-75</ispartof><rights>2017 Elsevier Inc.</rights><rights>Copyright © 2017 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c481t-dd20d4367253142921366c29285ddd1506c1b76b107c3bc057a5b9020a1bbf303</citedby><cites>FETCH-LOGICAL-c481t-dd20d4367253142921366c29285ddd1506c1b76b107c3bc057a5b9020a1bbf303</cites><orcidid>0000-0003-3384-6267 ; 0000-0003-2126-3013</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27911,27912</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/28456640$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Horas H Nababan, Saut</creatorcontrib><creatorcontrib>Nishiumi, Shin</creatorcontrib><creatorcontrib>Kawano, Yuki</creatorcontrib><creatorcontrib>Kobayashi, Takashi</creatorcontrib><creatorcontrib>Yoshida, Masaru</creatorcontrib><creatorcontrib>Azuma, Takeshi</creatorcontrib><title>Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD).
We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography/mass spectrometry (LC/MS). An in vitro experiment was performed using HepG2 cells stimulated with recombinant human TNFα or IL1β. The expression of steatosis-, inflammation-, and fibrosis-related genes were analyzed. Plasma samples from NAFLD patients were also analyzed by LC/MS.
The CDAHFD-fed db/db mice with hepatic steatosis, inflammation, mild fibrosis, obesity, and hypercholesterolemia displayed significantly higher hepatic and plasma levels of free adrenic acid (p < 0.05). The accumulated adrenic acid in the CDAHFD-fed db/db mice was associated with increased expression of ELOVL2 and 5, and the suppression of the acyl-CoA oxidase 1 gene during peroxisomal β-oxidation. The pretreatment of HepG2 cells with adrenic acid enhanced their cytokine-induced cytokines and chemokines mRNA expression. In NAFLD patients, the group with the highest ALT levels exhibited higher plasma adrenic acid concentrations than the other ALT groups (p-value for trend <0.001).
Data obtained demonstrated that adrenic acid accumulation contributes to disease progression in NAFLD.
•Plasma and hepatic levels of free adrenic acid were markedly increased in a mouse model of steatohepatitis.•Adrenic acid supplementation enhanced cytokine and chemokine gene expression in hepatocytes.•High ALT levels were associated with higher plasma adrenic acid levels in NAFLD patients.</description><subject>Adrenic acid</subject><subject>Adult</subject><subject>Alanine aminotransferase</subject><subject>Animals</subject><subject>Chemokine</subject><subject>Cytokines - analysis</subject><subject>Cytokines - immunology</subject><subject>Disease Models, Animal</subject><subject>Disease Progression</subject><subject>Fatty Acids, Unsaturated - analysis</subject><subject>Fatty Acids, Unsaturated - blood</subject><subject>Fatty Acids, Unsaturated - immunology</subject><subject>Female</subject><subject>Gene Expression Regulation</subject><subject>Hep G2 Cells</subject><subject>Humans</subject><subject>Inflammation - blood</subject><subject>Inflammation - genetics</subject><subject>Inflammation - immunology</subject><subject>Inflammation - pathology</subject><subject>Inflammation Mediators - analysis</subject><subject>Inflammation Mediators - blood</subject><subject>Inflammation Mediators - immunology</subject><subject>Lipids - analysis</subject><subject>Lipids - blood</subject><subject>Lipids - immunology</subject><subject>Liquid chromatography/mass-spectrometry</subject><subject>Liver - immunology</subject><subject>Liver - pathology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Middle Aged</subject><subject>Non-alcoholic fatty liver disease</subject><subject>Non-alcoholic Fatty Liver Disease - blood</subject><subject>Non-alcoholic Fatty Liver Disease - genetics</subject><subject>Non-alcoholic Fatty Liver Disease - immunology</subject><subject>Non-alcoholic Fatty Liver Disease - pathology</subject><subject>Oxidative Stress</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNp9kE1LxDAQhoMo7vrxA7xIj15aZ5o0bfAk4hcoXvQc8lU2S5tq0hX892ZZ9ehpYOZ5X5iHkDOECgH55bpSWlc1YFsBqwDEHlkiCF4C7dg-WQIALUXHcUGOUloDIDJeH5JF3bGGcwZL8nxtowveFMp4W6hUqFD40A9qHNXsp1C4sFLBuJi3RZhCqQYzraYhJ3o1z1_F4D_z0frkVHIn5KBXQ3KnP_OYvN3dvt48lE8v948310-lYR3OpbU1WEZ5WzcUWS1qpJybPLvGWosNcIO65RqhNVQbaFrVaAE1KNS6p0CPycWu9z1OHxuXZjn6ZNwwqOCmTZLYCSq4EI3IKO5QE6eUouvle_Sjil8SQW4tyrXMFuXWogQms8WcOf-p3-jR2b_Er7YMXO0Al5_89C7KZLzLmqyPzszSTv6f-m86noCy</recordid><startdate>20170601</startdate><enddate>20170601</enddate><creator>Horas H Nababan, Saut</creator><creator>Nishiumi, Shin</creator><creator>Kawano, Yuki</creator><creator>Kobayashi, Takashi</creator><creator>Yoshida, Masaru</creator><creator>Azuma, Takeshi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3384-6267</orcidid><orcidid>https://orcid.org/0000-0003-2126-3013</orcidid></search><sort><creationdate>20170601</creationdate><title>Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease</title><author>Horas H Nababan, Saut ; Nishiumi, Shin ; Kawano, Yuki ; Kobayashi, Takashi ; Yoshida, Masaru ; Azuma, Takeshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c481t-dd20d4367253142921366c29285ddd1506c1b76b107c3bc057a5b9020a1bbf303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>Adrenic acid</topic><topic>Adult</topic><topic>Alanine aminotransferase</topic><topic>Animals</topic><topic>Chemokine</topic><topic>Cytokines - analysis</topic><topic>Cytokines - immunology</topic><topic>Disease Models, Animal</topic><topic>Disease Progression</topic><topic>Fatty Acids, Unsaturated - analysis</topic><topic>Fatty Acids, Unsaturated - blood</topic><topic>Fatty Acids, Unsaturated - immunology</topic><topic>Female</topic><topic>Gene Expression Regulation</topic><topic>Hep G2 Cells</topic><topic>Humans</topic><topic>Inflammation - blood</topic><topic>Inflammation - genetics</topic><topic>Inflammation - immunology</topic><topic>Inflammation - pathology</topic><topic>Inflammation Mediators - analysis</topic><topic>Inflammation Mediators - blood</topic><topic>Inflammation Mediators - immunology</topic><topic>Lipids - analysis</topic><topic>Lipids - blood</topic><topic>Lipids - immunology</topic><topic>Liquid chromatography/mass-spectrometry</topic><topic>Liver - immunology</topic><topic>Liver - pathology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Middle Aged</topic><topic>Non-alcoholic fatty liver disease</topic><topic>Non-alcoholic Fatty Liver Disease - blood</topic><topic>Non-alcoholic Fatty Liver Disease - genetics</topic><topic>Non-alcoholic Fatty Liver Disease - immunology</topic><topic>Non-alcoholic Fatty Liver Disease - pathology</topic><topic>Oxidative Stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Horas H Nababan, Saut</creatorcontrib><creatorcontrib>Nishiumi, Shin</creatorcontrib><creatorcontrib>Kawano, Yuki</creatorcontrib><creatorcontrib>Kobayashi, Takashi</creatorcontrib><creatorcontrib>Yoshida, Masaru</creatorcontrib><creatorcontrib>Azuma, Takeshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Horas H Nababan, Saut</au><au>Nishiumi, Shin</au><au>Kawano, Yuki</au><au>Kobayashi, Takashi</au><au>Yoshida, Masaru</au><au>Azuma, Takeshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>2017-06-01</date><risdate>2017</risdate><volume>623-624</volume><spage>64</spage><epage>75</epage><pages>64-75</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><abstract>This study was designed to identify novel links between lipid species and disease progression in non-alcoholic fatty liver disease (NAFLD).
We analyzed lipid species in the liver and plasma of db/db mice fed a choline-deficient l-amino acid-defined, high-fat diet (CDAHFD) using liquid chromatography/mass spectrometry (LC/MS). An in vitro experiment was performed using HepG2 cells stimulated with recombinant human TNFα or IL1β. The expression of steatosis-, inflammation-, and fibrosis-related genes were analyzed. Plasma samples from NAFLD patients were also analyzed by LC/MS.
The CDAHFD-fed db/db mice with hepatic steatosis, inflammation, mild fibrosis, obesity, and hypercholesterolemia displayed significantly higher hepatic and plasma levels of free adrenic acid (p < 0.05). The accumulated adrenic acid in the CDAHFD-fed db/db mice was associated with increased expression of ELOVL2 and 5, and the suppression of the acyl-CoA oxidase 1 gene during peroxisomal β-oxidation. The pretreatment of HepG2 cells with adrenic acid enhanced their cytokine-induced cytokines and chemokines mRNA expression. In NAFLD patients, the group with the highest ALT levels exhibited higher plasma adrenic acid concentrations than the other ALT groups (p-value for trend <0.001).
Data obtained demonstrated that adrenic acid accumulation contributes to disease progression in NAFLD.
•Plasma and hepatic levels of free adrenic acid were markedly increased in a mouse model of steatohepatitis.•Adrenic acid supplementation enhanced cytokine and chemokine gene expression in hepatocytes.•High ALT levels were associated with higher plasma adrenic acid levels in NAFLD patients.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>28456640</pmid><doi>10.1016/j.abb.2017.04.009</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-3384-6267</orcidid><orcidid>https://orcid.org/0000-0003-2126-3013</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adrenic acid Adult Alanine aminotransferase Animals Chemokine Cytokines - analysis Cytokines - immunology Disease Models, Animal Disease Progression Fatty Acids, Unsaturated - analysis Fatty Acids, Unsaturated - blood Fatty Acids, Unsaturated - immunology Female Gene Expression Regulation Hep G2 Cells Humans Inflammation - blood Inflammation - genetics Inflammation - immunology Inflammation - pathology Inflammation Mediators - analysis Inflammation Mediators - blood Inflammation Mediators - immunology Lipids - analysis Lipids - blood Lipids - immunology Liquid chromatography/mass-spectrometry Liver - immunology Liver - pathology Male Mice Mice, Inbred C57BL Middle Aged Non-alcoholic fatty liver disease Non-alcoholic Fatty Liver Disease - blood Non-alcoholic Fatty Liver Disease - genetics Non-alcoholic Fatty Liver Disease - immunology Non-alcoholic Fatty Liver Disease - pathology Oxidative Stress |
| title | Adrenic acid as an inflammation enhancer in non-alcoholic fatty liver disease |
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