On the relationship between the physiological state of bacteria and rapid enzymatic assays of fecal coliforms in the environment

Culturable cells and non-culturable cells of fecal coliforms, obtained by irradiation at 312 nm were submitted to the combined stress conditions of salinity and starvation. After 14 days, beta-galactosidase activity of UV-irradiated cells was at least twice the value of non-irradiated cells. UV-irra...

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Bibliographic Details
Published in:Biotechnology letters 2003-08, Vol.25 (16), p.1329-1334
Main Authors: Villarino, Andrea, Toribio, Ana L, Brena, Beatriz M, Grimont, P A D, Bouvet, Odile M M
Format: Article
Language:English
Subjects:
Bacteria
beta-Galactosidase - analysis
beta-Galactosidase - metabolism
Colony Count, Microbial - methods
Dose-Response Relationship, Radiation
Enterobacteriaceae - classification
Enterobacteriaceae - enzymology
Enterobacteriaceae - growth & development
Enterobacteriaceae - radiation effects
Environmental Monitoring - methods
Enzyme Activation
Escherichia coli - enzymology
Escherichia coli - growth & development
Escherichia coli - isolation & purification
Escherichia coli - radiation effects
Fecal coliforms
Feces - microbiology
Glucose - metabolism
Irradiation
Microbiology
Saline water
Seawater - microbiology
Statistics as Topic
Ultraviolet Rays
Water Pollutants - analysis
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Summary:Culturable cells and non-culturable cells of fecal coliforms, obtained by irradiation at 312 nm were submitted to the combined stress conditions of salinity and starvation. After 14 days, beta-galactosidase activity of UV-irradiated cells was at least twice the value of non-irradiated cells. UV-irradiated cells thus contribute more than non-irradiated cells to the enzyme assay after incubation in saline water. This finding is essential for the interpretation of quantitative investigations into the environment using enzymatic methods.
ISSN:0141-5492
1573-6776
DOI:10.1023/A:1024903407025