p53 mutations in betel-associated oral cancer from Thailand

The p53 mutations in betel-related oral cancer have been studied but the results may be confounded by tobacco and alcohol due to tobacco components in the quid or concomitant habits of smoking and drinking. In this study, p53 mutations in tumors from Thai betel chewers who neither smoke nor drink we...

Full description

Saved in:
Bibliographic Details
Published in:Cancer letters 2003-11, Vol.201 (1), p.1-7
Main Authors: Thongsuksai, Paramee, Boonyaphiphat, Pleumjit, Sriplung, Hutcha, Sudhikaran, Wanna
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Alcohol Drinking - adverse effects
Antineoplastic agents
Areca - adverse effects
betel
betel chewing
Biological and medical sciences
Carcinoma, Squamous Cell - chemically induced
Carcinoma, Squamous Cell - genetics
Deoxyribonucleic acid
DNA
DNA Mutational Analysis
Female
Females
Genes, p53 - genetics
Glycerol
Humans
Male
Medical sciences
Middle Aged
Mouth Neoplasms - chemically induced
Mouth Neoplasms - genetics
Mutation
Oral cancer
p53
Pharmacology. Drug treatments
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Smoking
Smoking - adverse effects
Thailand
Tobacco, Smokeless - adverse effects
tumor suppressor gene
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The p53 mutations in betel-related oral cancer have been studied but the results may be confounded by tobacco and alcohol due to tobacco components in the quid or concomitant habits of smoking and drinking. In this study, p53 mutations in tumors from Thai betel chewers who neither smoke nor drink were studied in comparison to tumors from smokers/drinkers. PCR–SSCP analysis and direct sequencing on exon 5–8 were performed. Mutations were detected in 11.8% (8/68) of betel-related tumors and 7 of 8 mutations were G:C to A:T transitions. By contrast, mutations were found in 22.4% (13/58) of smoking/drinking-related tumors with various base substitutions. The results demonstrated different mutation profiles between the two exposed groups. The type of mutation detected in betel chewers suggested a possible role of areca-specific nitrosamine as a causative carcinogen.
ISSN:0304-3835
1872-7980
DOI:10.1016/S0304-3835(03)00417-8