Protective Effects of Acupuncture in Cardiopulmonary Bypass-Induced Lung Injury in Rats

Acute lung injury caused by cardiopulmonary bypass (CPB) increases the mortality after cardiac surgery. Our previous clinical study suggested that electroacupuncture (EAc) has a protective effect during CPB, but the mechanism was unclear. So, we design this study to investigate the effects of EAc on...

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Bibliographic Details
Published in:Inflammation 2017-08, Vol.40 (4), p.1275-1284
Main Authors: Ma, Wen, Li, Zigang, Lu, Zhou, Tan, Wenling, Zhang, Zhewen, Li, Yajun, Yang, Zhongwei, Zhou, Jia, Tang, Huifang, Cui, Huashun
Format: Article
Language:English
Subjects:
A549 Cells
Acupuncture
Animals
Apoptosis
Biomarkers - analysis
Biomedical and Life Sciences
Biomedicine
c-Jun protein
Cardiopulmonary Bypass - adverse effects
Caspase
Caspase-3
Electroacupuncture - methods
Extracellular signal-regulated kinase
Heart diseases
Heart surgery
Humans
Immunohistochemistry
Immunology
Inflammation - prevention & control
Internal Medicine
JNK protein
Lung Injury - pathology
Lung Injury - prevention & control
Lungs
Male
Malondialdehyde
Original Article
Oxidative Stress
Pathology
Peroxidase
Pharmacology/Toxicology
Phosphorylation
Rats
Rats, Sprague-Dawley
Rheumatology
Rodents
Superoxide dismutase
Transcription factors
Western blotting
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Summary:Acute lung injury caused by cardiopulmonary bypass (CPB) increases the mortality after cardiac surgery. Our previous clinical study suggested that electroacupuncture (EAc) has a protective effect during CPB, but the mechanism was unclear. So, we design this study to investigate the effects of EAc on CPB-induced lung injury and the underlying mechanism. Male Sprague Dawley rats were randomly divided into control, CPB, and CPB + EAc groups. A lung injury model was created by CPB surgery to serve as the CPB group, and EAc (2/100 Hz) was used before CPB in the CPB + EAc group. Lung tissue was collected at 0.5, 1, and 2 h after CPB. Pulmonary malondialdehyde (MDA) concentrations as well as superoxide dismutase (SOD), myeloperoxidase (MPO), and caspase-3 activity were determined. c-Jun N-terminal kinase (JNK), ERK, p38 and cleaved caspase 3 in the lung were analyzed by western blotting. A549 cells were treated by rat serum from the CPB and CPB + EAc groups, and cleaved caspase-3 activity was detected by fluorescent immunohistochemistry. CPB significantly increased the MPO activity, MDA content, apoptosis, caspase-3 activity, and phosphorylated p38 but decreased SOD activity compared with the control group. EAc significantly increased SOD activity at 0.5 and 2 h ( p  
ISSN:0360-3997
1573-2576
DOI:10.1007/s10753-017-0570-0