Autophagy-related gene microarray and bioinformatics analysis for ischemic stroke detection

• Published in: Biochemical and biophysical research communications 2017-07, Vol.489 (1), p.48-55
• Main Authors: Guo, Yinsheng, Ma, Yue, Zhang, Yanwei, Zhou, Li, Huang, Suli, Wen, Ying, Zou, Fei, Cheng, Jinquan
• Format: Article
• Language: English
• Subjects: Aged; Autophagy; Autophagy - genetics; Brain Ischemia - genetics; Brain Ischemia - pathology; Case-Control Studies; Case-control study; Computational Biology; Female; Gene Expression Profiling; Gene microarray; GO analysis; Humans; Ischemic stroke; Male; Middle Aged; Pathway analysis; Polymerase Chain Reaction; Stroke - genetics; Stroke - pathology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2017.05.099

Ischemic stroke (IS) is characterized by high morbidity and poor prognosis. However, the mechanisms of IS induced injury are still poorly understood. The main aim of this study is to explore the role of autophagy in IS. Ten pairs of whole blood samples of IS patients and matched controls were included to select differential expressed genes (DE genes) by autophagy-related functional gene microarray analysis. And then, one hundred and fifty pairs of whole blood samples of IS patients and matched controls were included to validate the DE genes. Moreover, Gene Ontology (GO) analyses and Pathway analyses were also performed based on the DE gene results. Our results indicated that the co-regulation of autophagy and apoptosis took part in IS-induced injuries, and mitochondrial autophagy and apoptosis played a crucial role in this process. Furthermore, lysosome, protein kinase and endopeptidase also participated in IS. These findings clarified the role of mitochondrial autophagy and apoptosis in ischemic stroke and provided more important biomarkers for the prevention diagnosis and therapeutic implications in IS. •Blood samples were used to identify biomarkers of IS with autophagy microarray.•Results of GO analysis showed that the prominent cellular component was lysosome.•Protein kinase and endopeptidase were the prominent molecular functions.•Apoptosis pathway was the most enriched pathway.•Mito-autophagy and apoptosis play a crucial role in IS-induced injury.