Cell-Free DNA, Inflammation, and the Initiation of Spontaneous Term Labor

Abstract Background Hypomethylated cell-free DNA (cfDNA) from senescent placental trophoblasts may be involved in the activation of the inflammatory cascade to initiate labor. Objective To determine the changes in cfDNA concentrations, the methylation ratio, and inflammatory markers between women in...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2017-11, Vol.217 (5), p.583.e1-583.e8
Main Authors: Herrera, Christina A., MD, Stoerker, Jay, PhD, Carlquist, John, PhD, Stoddard, Gregory J., MS, Jackson, Marc, MD, Esplin, Sean, MD, Rose, Nancy C., MD
Format: Article
Language:English
Subjects:
Adult
Case-Control Studies
Cellular Senescence
cfDNA
Cohort Studies
cytokines
DNA - blood
DNA - metabolism
DNA Methylation
Female
Fetus - metabolism
Gestational Age
Humans
IL-6
Inflammation
Interleukin-6 - immunology
Labor, Obstetric - immunology
Labor, Obstetric - metabolism
Longitudinal Studies
Obstetrics and Gynecology
Pregnancy
Pregnancy Trimester, Third
Prospective Studies
signals for labor
Term Birth
TLR9
Trophoblasts
Young Adult
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Summary:Abstract Background Hypomethylated cell-free DNA (cfDNA) from senescent placental trophoblasts may be involved in the activation of the inflammatory cascade to initiate labor. Objective To determine the changes in cfDNA concentrations, the methylation ratio, and inflammatory markers between women in labor at term versus women without labor. Study Design In this prospective cohort study, eligible participants carried a non- anomalous singleton fetus. Women with major medical comorbidity, preterm labor, progesterone use, aneuploidy, infectious disease, vaginal bleeding, abdominal trauma, or invasive procedures during the pregnancy were excluded. Maternal blood samples were collected at 28 weeks, 36 weeks, and at admission for delivery. Total cfDNA concentration, methylation ratio, and interleukin-6 (IL-6) were analyzed. The primary outcome was the difference in methylation ratio in women with labor versus without labor. Secondary outcomes included the longitudinal changes in these biomarkers corresponding to labor status. Results 55 women were included; 20 presented in labor on admission and 35 presented without labor. Women in labor had significantly higher methylation ratio (p= 0.001) and IL-6 (p
ISSN:0002-9378
1097-6868
DOI:10.1016/j.ajog.2017.05.027