High throughput and automatic colony formation assay based on impedance measurement technique

To predict the response of in vivo tumors, in vitro culture of cell colonies was suggested to be a standard assay to achieve high clinical relevance. To describe the responses of cell colonies, the most widely used quantification method is to count the number and size of cell colonies under microsco...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-05, Vol.409 (12), p.3271-3277
Main Authors: Lei, Kin Fong, Kao, Chich-Hao, Tsang, Ngan-Ming
Format: Article
Language:English
Subjects:
Analysis
Analytical Chemistry
Assaying
Automation
Biochemistry
Cancer
Cancer research
Cell culture
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Colonies
Culture
Electrodes
Food Science
Formations
Hydrogels
Impedance
Impedance measurement
Laboratory Medicine
Measurement techniques
Medical research
Microscopes
Monitoring/Environmental Analysis
Research Paper
Tumors
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Summary:To predict the response of in vivo tumors, in vitro culture of cell colonies was suggested to be a standard assay to achieve high clinical relevance. To describe the responses of cell colonies, the most widely used quantification method is to count the number and size of cell colonies under microscope. That makes the colony formation assay infeasible to be high throughput and automated. In this work, in situ analysis of cell colonies suspended in soft hydrogel was developed based on impedance measurement technique. Cell colonies cultured between a pair of parallel plate electrodes were successfully analyzed by coating a layer of base hydrogel on one side of electrode. Real-time and label-free monitoring of cell colonies was realized during the culture course. Impedance magnitude and phase angle respectively represented the summation effect of colony responses and size of colonies. In addition, dynamic response of drug-treated colonies was demonstrated. High throughput and automatic colony formation assay was realized to facilitate more objective assessments in cancer research. Graphical Abstract High throughput and automatic colony formation assay was realized by in situ impedimetric analysis across a pair of parallel plate electrodes in a culture chamber. Cell colonies suspended in soft hydrogel were cultured under the tested substance and their dynamic response was represented by impedance data.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-017-0270-5