Anticonvulsive activity of (1S)-(−)-verbenone involving RNA expression of BDNF, COX-2, and c-fos

(1S)-(−)-verbenone (VRB) is a monoterpene present in the essential oils of many plants which has shown therapeutic effect; however, its anticonvulsant activity has not yet been evaluated. The present work sought to investigate the anticonvulsant activity of VRB using pilocarpine and pentylenetetrazo...

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Bibliographic Details
Published in:Naunyn-Schmiedeberg's archives of pharmacology 2017-09, Vol.390 (9), p.863-869
Main Authors: de Melo, Cynthia Germoglio Farias, Salgado, Paula Regina Rodrigues, da Fonsêca, Diogo Vilar, Braga, Renan Marinho, Filho, Marcelo Ricardo Dutra Caldas, de Farias, Ingrid Eulália Vieira, de Luna Freire Pessôa, Hilzeth, Lima, Eleonidas Moura, do Amaral, Ian Porto Gurgel, de Sousa, Damião Pergentino, de Almeida, Reinaldo Nóbrega
Format: Article
Language:English
Subjects:
Animals
Anticonvulsants
Biomedical and Life Sciences
Biomedicine
Brain-derived neurotrophic factor
c-Fos protein
Convulsions & seizures
Cyclooxygenase-2
Drug dosages
Epilepsy
Essential oils
Flumazenil
Gene expression
Kinases
Latency
Motor ability
Motor task performance
Nervous system
Neurosciences
Oils & fats
Original Article
Pentylenetetrazole
Pharmacology/Toxicology
Pilocarpine
Ribonucleic acid
RNA
Seizing
Seizures
Verbenone
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Summary:(1S)-(−)-verbenone (VRB) is a monoterpene present in the essential oils of many plants which has shown therapeutic effect; however, its anticonvulsant activity has not yet been evaluated. The present work sought to investigate the anticonvulsant activity of VRB using pilocarpine and pentylenetetrazole-induced seizure testing; seeking also probable mechanisms of action. VRB caused no significant changes in motor coordination. Also, no significant data was observed in the pilocarpine-induced seizure tests. In the PTZ-induced seizures test, VRB showed anticonvulsant activity at doses of 200 mg/kg i.p. (733 ± 109.4 s) and 250 mg/kg i.p. (648.8 ± 124.5 s) significantly increasing the latency to onset of first seizure as compared with the vehicle group (51.8 ± 2.84 s). Pretreatment with flumazenil (FLU) did not reverse the anticonvulsive effect of VRB; however, it was able to upregulate BDNF and COX-2 genes and downregulate c-fos. The findings suggest that the anticonvulsant effects of VRB may be related to RNA expression modulations of COX-2, BDNF, and c-fos.
ISSN:0028-1298
1432-1912
DOI:10.1007/s00210-017-1388-x