Comparative morphology and phagocytic capacity of primary human adult microglia with time-lapse imaging

Abstract Microglia provide immune surveillance within the brain and spinal cord. Various microglial morphologies include ramified, amoeboid, and pseudopodic. The link between form and function is not clear, especially for human adult microglia which are limited in availability for study. Here, we ex...

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Bibliographic Details
Published in:Journal of neuroimmunology 2017-09, Vol.310, p.143-149
Main Authors: Levtova, Natalie, Healy, Luke M, Gonczi, Catalina Marysol Carvajal, Stopnicki, Brandon, Blain, Manon, Kennedy, Timothy E, Moore, Craig S, Antel, Jack P, Darlington, Peter J
Format: Article
Language:English
Subjects:
Actins - metabolism
Allergy and Immunology
Amoeba - cytology
Amoeboid
Antigens, CD - metabolism
Antigens, Differentiation, Myelomonocytic - metabolism
Bacterial Infections
Cells, Cultured
Dendritic cells
DNA-Binding Proteins - metabolism
E. coli bioparticles
Epilepsy - pathology
Escherichia coli - pathogenicity
Humans
Microglia - microbiology
Microglia - pathology
Microglia - physiology
Neurology
Phagocytes - cytology
Phagocytes - physiology
Primary human adult microglia
Pseudopodic
Ramified
Temporal Lobe - pathology
Time Factors
Time lapse microscopy
Time-Lapse Imaging
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Summary:Abstract Microglia provide immune surveillance within the brain and spinal cord. Various microglial morphologies include ramified, amoeboid, and pseudopodic. The link between form and function is not clear, especially for human adult microglia which are limited in availability for study. Here, we examined primary human microglia isolated from normal-appearing white matter. Pseudopodic and amoeboid microglia were effective phagocytes, taking up E. coli bioparticles using ruffled cell membrane sheets and retrograde transport. Pseudopodic and amoeboid microglia were more effective phagocytes as compared to ramified microglia or monocyte-derived dendritic cells. Thus, amoeboid and pseudopodic microglia may both be effective as brain scavengers.
ISSN:0165-5728
1872-8421
DOI:10.1016/j.jneuroim.2017.05.012