P42Association of c677t polymorphism of mthfr gene with hemostasis changes in adolescents with essential hypertension

• Published in: Archives of disease in childhood 2017-06, Vol.102 (Suppl 2), p.A51-A51
• Main Authors: Lubov, Rychkova, Svetlana, Bolshakova, Marina, Gomellya, Tatyana, Bairova, Olga, Berdina, Olga, Bugun
• Format: Article
• Language: English
• ISSN: 0003-9888
• DOI: 10.1136/archdischild-2017-313273.130

Background and aimsIt is known that thrombosis in adolescents with essential hypertension (EH) are rare, however pro-coagulation hemostatic changes are multifactorial and requires detailed study. One of the risk factors can be C677T polymorphism of MTHFR gene, which changes of homocysteine metabolism, rendering thrombogenic effect. Thus, the aim of this study is to study association of C677T polymorphism of MTHFR gene with hemostasis parameters in hypertensive adolescents with and without protrombotic changes (PTC).MethodsWe examined 97 Caucasian adolescents with EH, living in Eastern Siberia, aged 14-17 years. Demographic and anthropometric data were routinely collected. Hemostatic study was performed applying auto-coagulometr STA-R Evolution (Roche Diagnostics, Switzerland). Genetic polymorphism detection was carried out using polymerase chain reaction with specific primers (, Litex, Russia). All patients were selected for participation in this study based on their coagulation activity and C677T genotype. The 1-rst group - 29 adolescents with PTC and C/T and T/T genotypes; the 2-nd group - 8 patients with PTC and C/C genotypes; the 3-rd group - 28 adolescents without PTC and with C/T and T/T genotypes; the 4-th group - 32 patients without PTC and with C/C genotypes. All differences were considered significant at p