Recovery evaluation of rats' damaged tibias: Implantation of core-shell structured bone scaffolds made using hollow braids and a freeze-thawing process

• Published in: Materials Science & Engineering C 2017-10, Vol.79, p.481-490
• Main Authors: Lin, Jia-Horng, Lee, Mong-Chuan, Chen, Chih-Kuang, Huang, Chien-Lin, Chen, Yueh-Sheng, Wen, Shih-Peng, Kuo, Shu-Ting, Lou, Ching-Wen
• Format: Article
• Language: English
• Subjects: Alcohols; Animals; Biodegradability; Biodegradable materials; Biodegradation; Biomedical materials; Bone and Bones; Bone scaffolds; Braid; Braiding; Compression; Compressive strength; Core-shell structure; Damage assessment; Durapatite; Freeze drying; Freeze-thawing; Freezing; Gelatin; Hydroxyapatite; Hydroxyapatite (HA); Implantation; Infiltration; Materials science; Mechanical properties; Melting; Morphology; Ossification; Polyvinyl alcohol; Polyvinyl alcohol (PVA); Porosity; Rats; Recovery; Recovery of damaged bone; Rodents; Scaffolds; Structural damage; Surgical implants; Thawing; Tibia; Tissue Engineering; Tissue Scaffolds; Yarns
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2017.04.156

This study prepares biodegradable bone scaffolds helping the recovery of damaged tibias of rats. Polyvinyl alcohol (PVA) plied yarns are fabricated into hollow braids. The braids are combined with hydroxyapatite (HA)/gelatin/PVA mixtures and processed using freeze-thawing and freeze-drying processes in order to form bone scaffolds. These bone scaffolds are observed by scanning electron scope (SEM) and tested for compression strength. Afterwards, recovery of damaged bone, the morphology of the bone, and the histological observation are evaluated. Results indicate a small amount of HA helps in enhancing the compressive strength of bone scaffolds. Results of in vivo assay indicate the damaged tibias of rats recover and function well eight weeks after the implantation, and exhibit a normal morphology. Histological observation confirms the bone scaffolds gradually decompose, allowing tissue infiltration and facilitating ossification. This study successfully produces bone scaffolds with satisfactory mechanical properties helping in the recovery of damaged tibias of rats. •A braiding technique is incorporated with the production of bone scaffolds.•Morphology, histological observation, and the rats' walking ability are evaluated.•With bone scaffolds, rats' damaged tibias recover, thereby enabling them to walk.