Genome-wide Targeted Mutagenesis in Rice Using the CRISPR/Cas9 System

• Published in: Molecular plant 2017-09, Vol.10 (9), p.1242-1245
• Main Authors: Lu, Yuming, Ye, Xiao, Guo, Renming, Huang, Jing, Wang, Wei, Tang, Jiuyou, Tan, Longtao, Zhu, Jian-kang, Chu, Chengcai, Qian, Yangwen
• Format: Article
• Language: English
• Subjects: Base Sequence; CRISPR-Cas Systems - genetics; Genome, Plant - genetics; Mutagenesis - genetics; 功能鉴定; 基因位点; 基因突变; 基因组测序; 定向诱变; 水稻育种; 生物学过程; 系统
• ISSN: 1674-2052; 1752-9867
• DOI: 10.1016/j.molp.2017.06.007

Dear Editor Since the completion of the rice （Oryza sativa） genome- sequencing project, a major goal of rice research has been the functional characterization of all annotated genetic loci in various biological processes. One of the most efficient and widely-used strategies for studying gene function is genetic mutagenesis. Several rice mutant libraries have been generated in the past decade, providing a wealth of resources for plant research （Chang et al., 2012）. CRISPR/Cas9 （clustered regularly interspaced short palindromic repeats-associated nuclease 9） has recently emerged as a powerful tool for rice research and breeding （Cong et al., 2013; Feng et al., 2013; Miao et al., 2013;