Phytol Promotes the Formation of Slow-Twitch Muscle Fibers through PGC-1α/miRNA but Not Mitochondria Oxidation

• Published in: Journal of agricultural and food chemistry 2017-07, Vol.65 (29), p.5916-5925
• Main Authors: Yang, Kelin, Wang, Lina, Zhou, Gan, Lin, Xiajing, Peng, Jianlong, Wang, Leshan, Luo, Lv, Wang, Jianbin, Shu, Gang, Wang, Songbo, Gao, Ping, Zhu, Xiaotong, Xi, Qianyun, Zhang, Yongliang, Jiang, Qingyan
• Format: Article
• Language: English
• Subjects: Animals; Cell Line; Male; Mice; MicroRNAs - genetics; MicroRNAs - metabolism; Mitochondria - genetics; Mitochondria - metabolism; Muscle Fibers, Skeletal - metabolism; Muscle Fibers, Slow-Twitch - metabolism; Oxidation-Reduction; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - genetics; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha - metabolism; Phytol - metabolism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.7b01048

Phytol is a side chain of chlorophyll belonging to the side-chain double terpenoid. When animals consume food rich in chlorophyll, phytol can be broken down to phytanic acid after digestion. It was reported that feeding animals with different varieties and levels of forage could significant improve pH and marbling score of steer and lamb carcasses, but the internal mechanism for this is still not reported. The marbling score and pH of muscle was mainly determined by skeletal muscle fiber type, which is due to expression of different myosin heavy-chain (MHC) isoforms. Here, we provide evidence that phytol can indeed affect the diversity of muscle fiber types both in vitro and in vivo and demonstrate that phytol can increase the expression of MHC I (p < 0.05), likely by upgrading the expression of PPARδ, PGC-1α, and related miRNAs. This fiber-type transformation process may not be caused by activated mitochondrial metabolism but by the structural changes in muscle fiber types.