Caspase activation during Haemophilus somnus lipooligosaccharide-mediated apoptosis of bovine endothelial cells

Vasculitis is commonly seen during systemic Haemophilus somnus infections. Although, the mechanism of vascular damage is not completely understood, in a previous report we demonstrated that H. somnus and its lipooligosaccharide (LOS) induced apoptosis in bovine pulmonary artery endothelial cells in...

Full description

Saved in:
Bibliographic Details
Published in:Microbial pathogenesis 2003-12, Vol.35 (6), p.285-291
Main Authors: Sylte, Matt J, Leite, Fabio P, Kuckleburg, Chris J, Inzana, Thomas J, Czuprynski, Charles J
Format: Article
Language:English
Subjects:
Amino Acid Chloromethyl Ketones - pharmacology
Animals
Apoptosis
Bacteriology
Biological and medical sciences
Caspase
Caspase 3
Caspase 8
Caspase 9
Caspase Inhibitors
Caspases - metabolism
Cattle
Cell Line
Chromatin - pathology
Endothelial cell
Endothelium, Vascular - cytology
Endothelium, Vascular - enzymology
Endothelium, Vascular - physiology
Enzyme Activation
Enzyme Inhibitors - pharmacology
Fundamental and applied biological sciences. Psychology
Haemophilus somnus
Haemophilus somnus - immunology
Haemophilus somnus - pathogenicity
Lipooligosaccharide
lipooligosaccharides
Lipopolysaccharides - toxicity
Microbiology
Miscellaneous
Oligopeptides - pharmacology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vasculitis is commonly seen during systemic Haemophilus somnus infections. Although, the mechanism of vascular damage is not completely understood, in a previous report we demonstrated that H. somnus and its lipooligosaccharide (LOS) induced apoptosis in bovine pulmonary artery endothelial cells in vitro. In the present study, we investigated the role of caspase activation in LOS-mediated apoptosis of bovine endothelial cells. Exposure to H. somnus LOS induced caspase-3 activation and chromatin condensation in endothelial cells. These responses were blocked by the addition of a pan-caspase inhibitor (z-VAD-fmk) or capase-3 inhibitor (DEVD-fmk). Incubation of endothelial cells with H. somnus LOS also induced activation of the initiator caspases, caspases-8 and 9, with the activity of the former increasing more rapidly than the latter. Addition of a caspase-8 inhibitor (IETD-fmk) significantly reduced LOS-mediated apoptosis, whereas, addition of a caspase-9 inhibitor (LEHD-fmk) had little effect. These data suggest that LOS-mediated activation of caspase-3 and apoptosis of endothelial cells is caspase-8 dependent.
ISSN:0882-4010
1096-1208
DOI:10.1016/j.micpath.2003.08.002