Oral Administration of β-Lactamase Preserves Colonization Resistance of Piperacillin-Treated Mice

We hypothesized that orally administered, recombinant class A β-lactamase would inactivate the portion of parenteral piperacillin excreted into the intestinal tract, preserving colonization resistance of mice against nosocomial pathogens. Subcutaneous piperacillin or piperacillin plus oral β-lactama...

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Bibliographic Details
Published in:The Journal of infectious diseases 2003-11, Vol.188 (10), p.1605-1609
Main Authors: Stiefel, Usha, Pultz, Nicole J., Harmoinen, Jaana, Koski, Pertti, Lindevall, Kai, Helfand, Marion S., Donskey, Curtis J.
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacokinetics
Anti-Bacterial Agents - pharmacology
Antibiotics
beta -Lactamase
beta-Lactam Resistance - drug effects
beta-Lactamases - pharmacology
Biological and medical sciences
Candida glabrata - genetics
Candida glabrata - metabolism
Candidiasis - drug therapy
Community-Acquired Infections - drug therapy
Cross Infection - drug therapy
DNA, Bacterial - chemistry
DNA, Bacterial - genetics
DNA, Fungal - chemistry
DNA, Fungal - genetics
Dosage
Drug Synergism
Enterococcus faecium - genetics
Enterococcus faecium - metabolism
Enzymes
Feces - microbiology
Female
Fundamental and applied biological sciences. Psychology
Gels
Gram-Positive Bacterial Infections - drug therapy
Health care administration
Infectious diseases
Klebsiella Infections - drug therapy
Klebsiella pneumoniae - genetics
Klebsiella pneumoniae - metabolism
Medical sciences
Mice
Microbial colonization
Microbiology
Microorganisms
Oral administration
Pathogens
piperacillin
Piperacillin - pharmacokinetics
Piperacillin - pharmacology
Polymerase Chain Reaction
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Summary:We hypothesized that orally administered, recombinant class A β-lactamase would inactivate the portion of parenteral piperacillin excreted into the intestinal tract, preserving colonization resistance of mice against nosocomial pathogens. Subcutaneous piperacillin or piperacillin plus oral β-lactamase were administered 24 and 12 h before orogastric inoculation of piperacillin-resistant pathogens. Oral administration of β-lactamase reduced piperacillin-associated alteration of the indigenous microflora and prevented overgrowth of pathogens
ISSN:0022-1899
1537-6613
DOI:10.1086/379153