Detection of multidrug resistant (MDR) bacteria in untreated waste water disposals of hospitals in Dhaka City, Bangladesh

• Published in: Journal of global antimicrobial resistance. 2017-09, Vol.10, p.120-125
• Main Authors: Rabbani, M.A.G., Howlader, Md. Zakir Hossain, Kabir, Yearul
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Bacteria - drug effects; Bacteria - isolation & purification; Bangladesh; Drug Resistance, Multiple, Bacterial - drug effects; E. coli; Escherichia coli - drug effects; Escherichia coli - isolation & purification; Hospital waste; Hospitals; Humans; K. pneumoniae; Klebsiella pneumoniae - drug effects; Klebsiella pneumoniae - isolation & purification; Microbial Sensitivity Tests; Multidrug resistant; Waste Water - microbiology
• ISSN: 2213-7165; 2213-7173
• DOI: 10.1016/j.jgar.2017.04.009

•Highly resistant E. coli and K. pneumoniae isolates were detected in hospital waste water.•Highly resistant E. coli isolates confer resistance to additional broad spectrum antibiotics.•The sites located near the disposal point of hospital waste contained higher degree of resistant isolate than the distant ones. Emergence of MDR bacteria is a global problem and a major burden for treatment of various infectious diseases. This study was performed to detect antibiotic resistant bacteria in untreated hospital waste. Waste water samples were collected from sewerage disposal points of two renowned hospital of Dhaka city and a total of 59 Escherichia coli and 29 Klebsiella pneumonia isolates were isolated. Antibiotic susceptibility were measured by disc diffusion method. Resistance among E. coli and K. pneumoniae to 10 frequently used antibiotic tested were respectively as follows: cefotaxime (CTX) 48 and 45%; ceftazidime (CAZ) 40 and 38%; ampicillin (AMP) 71 and 100%; streptomycin (S) 50 and 34%; sulfamethoxazole-trimethoprim (SXT) 58 and 28%; ciprofloxacin (CIP) 71 and 48%; kanamycin (K) 38 and 10%; chloramphenicol (C) 28 and 10%; gentamycin (CN) 19 and 16% and imipenem (IPM) 12 and 7%. Results also demonstrate that the sites that were at the disposal point of hospital waste have higher degree of resistance. High degree of resistance was observed when 23 high-resistant E. coli isolates were further tested with 15 additional antibiotics. This study revealed a significant rise of MDR bacteria in the hospital waste and underscore necessity of hospital waste treatment.