Transposon-free insertions for insect genetic engineering

Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable trans...

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Bibliographic Details
Published in:Nature biotechnology 2006-07, Vol.24 (7), p.820-821
Main Authors: Dafa'alla, T.H, Condon, G.C, Condon, K.C, Phillips, C.E, Morrison, N.I, Jin, L, Epton, M.J, Fu, G, Alphey, L
Format: Article
Language:English
Subjects:
Agriculture
Animals
Animals, Genetically Modified - genetics
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
brief-communication
Ceratitis capitata
Ceratitis capitata - genetics
Disease control
DNA Transposable Elements - genetics
Fundamental and applied biological sciences. Psychology
Gene Deletion
gene transfer
Genetic engineering
Genetic Engineering - methods
Genetic technics
genetic transformation
genetic vectors
Genetic Vectors - genetics
Genomic Instability - genetics
Insects
Life Sciences
Methods. Procedures. Technologies
Mutagenesis, Site-Directed - methods
Pest control
Pests
post-integration transposon removal
Tephritidae
Transgenic animals
Transgenic animals and transgenic plants
transgenic insects
Transposases - genetics
transposon-mediated insertions
transposons
Vector-borne diseases
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Description
Summary:Methods involving the release of transgenic insects in the field hold great promise for controlling vector-borne diseases and agricultural pests. Insect transformation depends on nonautonomous transposable elements as gene vectors. The resulting insertions are stable in the absence of suitable transposase, however, such absence cannot always be guaranteed. We describe a method for post-integration elimination of all transposon sequences in the pest insect Medfly, Ceratitis capitata. The resulting insertions lack transposon sequences and are therefore impervious to transposase activity.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt1221