Exploration of genes that encode a carbocycle-forming enzyme involved in biosynthesis of aminoglycoside antibiotics from the environmental DNA

2-Deoxy-scyllo-inosose (DOI) synthase is the enzyme participating in biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotics. The gene which encodes the enzyme can be a marker for screening of DOS-containing aminoglycoside-producer and exploration of its biosynthetic gene. Fur...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2006-07, Vol.70 (7), p.1711-1716
Main Authors: Tamegai, H.(Nihon Univ., Tokyo (Japan)), Kuki, T, Udagawa, Y, Aoki, R, Nagaya, A, Tsukada, S
Format: Article
Language:English
Subjects:
2-deoxy-scyllo-inosose synthase
Actinobacteria - enzymology
Amino Acid Sequence
aminoglycoside
Aminoglycosides - biosynthesis
Anti-Bacterial Agents - biosynthesis
ANTIBIOTICOS
ANTIBIOTICS
ANTIBIOTIQUE
Biological and medical sciences
BIOSINTESIS
BIOSYNTHESE
BIOSYNTHESIS
biosynthetic gene
DNA, Bacterial - genetics
DNA, Bacterial - metabolism
DNA, Fungal - genetics
DNA, Fungal - metabolism
environmental DNA
Fundamental and applied biological sciences. Psychology
GENE
GENES
Hexosamines
industrial use
LIASAS
LYASE
LYASES
Lyases - genetics
Lyases - metabolism
Molecular Sequence Data
Phylogeny
Polymerase Chain Reaction
Soil Microbiology
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Summary:2-Deoxy-scyllo-inosose (DOI) synthase is the enzyme participating in biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside antibiotics. The gene which encodes the enzyme can be a marker for screening of DOS-containing aminoglycoside-producer and exploration of its biosynthetic gene. Further, this enzyme is expected to be of use in industry, because it converts sugar into 6-membered carbocycle. In the present study, we identified 21 clones encoding DOI synthase from environmental DNA by degenerate PCR. They were clearly divided into two groups. One appeared to derive from actinomycetes, and the other from non-actinomycetes. The latter group was larger (17 clones) than the former (four clones) despite the fact that only one strain of non-actinomycete was identified for DOS-containing aminoglycoside production. This result indicates that there are still many unidentified non-actinomycetes for DOS-containing aminoglycoside biosynthesis. We showed the possibility of identification of novel aminoglycoside-producing non-actinomycete from soil, and for development of more efficient enzymes for industrial use.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.60045