Matrix influence on the optical response of composite bacteriorhodopsin films to ammonia

The practical fabrication of thin-film chemical sensors generally requires the responsive element to be embedded in a matrix. The influence of different organic and inorganic matrices (polyvinyl alcohol (PVA), gelatin, and silica) on the static and dynamic optical properties of bacteriorhodopsin (bR...

Full description

Saved in:
Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2008-07, Vol.133 (1), p.281-290
Main Authors: Korposh, S.O., Sharkan, Y.P., Sichka, M.Y., Yang, D.-H., Lee, S.-W., Ramsden, J.J.
Format: Article
Language:English
Subjects:
Ammonia
Bacteriorhodopsin
Gelatin
Gelatins
Hydroxyl groups
Matrices
Photocycle
Photoresponse
Polyvinyl alcohols
PVA
Silica gel
Silica sol–gel
Silicon dioxide
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The practical fabrication of thin-film chemical sensors generally requires the responsive element to be embedded in a matrix. The influence of different organic and inorganic matrices (polyvinyl alcohol (PVA), gelatin, and silica) on the static and dynamic optical properties of bacteriorhodopsin (bR)-containing films, including their responses to ammonia, were studied. Morphological and cross-sectional studies of the bR-containing films using atomic force microscopy, scanning electron microscopy, and energy dispersive spectroscopy revealed a uniform distribution of the bR within the films; the silica films were much rougher and more porous than those made with the polymers; gelatin tended to suppress the ammonia response (because of its amine groups); PVA and silica greatly slowed down the photocycle (because of their hydroxyl groups). The silica-based film had the fastest and most sensitive response to ammonia.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2008.02.038