Genome-wide DNA methylation variation in maternal and cord blood of gestational diabetes population

• Published in: Diabetes research and clinical practice 2017-10, Vol.132, p.127-136
• Main Authors: Kang, Jessica, Lee, Chien-Nan, Li, Hung-Yuan, Hsu, Kai-Han, Lin, Shin-Yu
• Format: Article
• Language: English
• Subjects: Adult; Diabetes mellitus; Diabetes, Gestational - blood; DNA methylation; DNA Methylation - genetics; Epigenesis, Genetic - genetics; Epigenetics; Female; Fetal Blood - metabolism; Genome-Wide Association Study - methods; Gestational diabetes; Humans; Infant, Newborn; Metabolic disease; Pregnancy
• ISSN: 0168-8227; 1872-8227
• DOI: 10.1016/j.diabres.2017.07.034

•Compare the whole-genome DNA methylation variation in GDM population.•Identified the top 200 loci with significant difference in methylation status.•GDM has epigenetic effects on both mother and their offspring. Gestational diabetes mellitus (GDM) has always been a concerning issue for pregnant women. In recent studies, GDM was found to be related to epigenetic modifications, which would alter gene expressions, thus affecting the patients’ and their offspring’s health, leading to a higher probability of developing metabolic syndromes and diabetes later in life. In this study, we collected both maternal and cord blood samples from 16 pregnant women and their newborns, including eight exposed to GDM. GDM was diagnosed via a 75g oral glucose tolerance test (OGTT) at 24–28weeks of pregnancy. DNA methylation was measured at 841,573 CpG sites via the Infinium HumanMethylationEPIC BeadChip. An Ingenuity Pathway Analysis was conducted afterwards to identify genes and pathways epigenetically affected by GDM. We identified the top 200 loci and their corresponding genes in the maternal blood group (n=151) and cord blood group (n=167), both of which were methylated differently in the GDM and unexposed group. Metabolic disease-related pathways and molecules, such as interleukin-6 and interleukin-10 were identified in both groups. These results suggested that GDM has epigenetic effects on both mother and their offspring, which might result in future metabolic syndromes or diabetes. The high-throughput platform enabled us to analyze methylation sites throughout the genome and identify the most promising genes and pathways associated with GDM.