Improving the catalytic characteristics of lipase-displaying yeast cells by hydrophobic modification

Lipase-displaying yeast cells are a promising alternative to the conventional immobilised lipases for organic bioconversions. However, the hydrophilic characteristics of the yeast cell surface may impede efficient immobilisation. Herein, we tested three methods to enhance the hydrophobicity of the s...

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Bibliographic Details
Published in:Bioprocess and biosystems engineering 2017-11, Vol.40 (11), p.1689-1699
Main Authors: Zhang, Kun, Jin, Zi, Wang, Pan, Zheng, Sui-Ping, Han, Shuang-Yan, Lin, Ying
Format: Article
Language:English
Subjects:
Biocatalysts
Biodiesel fuels
Bioengineering
Biofuels
Biotechnology
Candida - enzymology
Carbon sources
Catalysis
Cell surface
Chemical synthesis
Chemistry
Chemistry and Materials Science
Environmental Engineering/Biotechnology
Esterification
Fatty acids
Fermentation
Food Science
Fungi
Hydrophobic and Hydrophilic Interactions
Hydrophobicity
Immobilization
Industrial and Production Engineering
Industrial Chemistry/Chemical Engineering
Ionic liquids
Lipase
Lipase - metabolism
Lymphocytes B
Pichia - enzymology
Pichia pastoris
Research Paper
Substrate Specificity
Surface Properties
Yeast
Yeasts
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Summary:Lipase-displaying yeast cells are a promising alternative to the conventional immobilised lipases for organic bioconversions. However, the hydrophilic characteristics of the yeast cell surface may impede efficient immobilisation. Herein, we tested three methods to enhance the hydrophobicity of the surface of Candida antarctica lipase B-displaying Pichia pastoris cells, co-displaying a fungal hydrophobin, coating with ionic liquids, and adding decane as a hydrophobic carbon source during fermentation. Modified cells showed higher surface hydrophobicity and superior esterification of C 6 –C 18 saturated fatty acids in hydrophobic solvents. When used for biodiesel synthesis, modified cells exhibited an improved initial reaction rate and equilibrium fatty acid methyl ester yield. We systematically discuss the influence of cell surface hydrophobicity on the catalytic properties, and the results provide guidance for improving the catalytic efficiency and operational characteristics of lipase-displaying yeast cells for organic bioconversions.
ISSN:1615-7591
1615-7605
DOI:10.1007/s00449-017-1824-9