Dynamic proteome profiling of individual proteins in human skeletal muscle after a high‐fat diet and resistance exercise

• Published in: The FASEB journal 2017-12, Vol.31 (12), p.5478-5494
• Main Authors: Camera, Donny M., Burniston, Jatin G., Pogson, Mark A., Smiles, William J., Hawley, John A.
• Format: Article
• Language: English
• Subjects: Abundance; adaptation; Body weight; Breakdown; Carbohydrates; Contraction; Deuterium; Diet; Glucose; Heavy water; High fat diet; Males; Mass spectrometry; Mass spectroscopy; metabolism; Muscle contraction; muscle protein synthesis; Muscles; Musculoskeletal system; Overweight; Protein biosynthesis; protein degradation; Protein synthesis; Proteins; Proteomes; Skeletal muscle
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.201700531R

ABSTRACT It is generally accepted that muscle adaptation to resistance exercise (REX) training is underpinned by contraction‐induced, increased rates of protein synthesis and dietary protein availability. By using dynamic proteome profiling (DPP), we investigated the contribution of both synthesis and breakdown to changes in abundance on a protein‐by‐protein basis in human skeletal muscle. Age‐matched, overweight males consumed 9 d of a high‐fat, low‐carbohydrate diet during which time they either undertook 3 sessions of REX or performed no exercise. Precursor enrichment and the rate of incorporation of deuterium oxide into newly synthesized muscle proteins were determined by mass spectrometry. Ninety proteins were included in the DPP, with 28 proteins exhibiting significant responses to REX. The most common pattern of response was an increase in turnover, followed by an increase in abundance with no detectable increase in protein synthesis. Here, we provide novel evidence that demonstrates that the contribution of synthesis and breakdown to changes in protein abundance induced by REX differ on a protein‐by‐protein basis. We also highlight the importance of the degradation of individual muscle proteins after exercise in human skeletal muscle.—Camera, D. M., Burniston, J. G., Pogson, M. A., Smiles, W. J., Hawley, J. A. Dynamic proteome profiling of individual proteins in human skeletal muscle after a high‐fat diet and resistance exercise. FASEB J. 31, 5478–5494 (2017). www.fasebj.org