In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation

• Published in: Journal of biophotonics 2018-02, Vol.11 (2), p.n/a
• Main Authors: Sherlock, Ben, Warren, Sean C., Alexandrov, Yuriy, Yu, Fei, Stone, James, Knight, Jonathan, Neil, Mark A. A., Paterson, Carl, French, Paul M. W., Dunsby, Chris
• Format: Article
• Language: English
• Subjects: autofluorescence; Compensation; Curvature; Equipment Design; Fluorescence; Forearm - diagnostic imaging; Hand; Human motion; Humans; Image stabilizers; Imaging; Lasers; Medical imaging; Microscopy; Microscopy, Fluorescence, Multiphoton - instrumentation; Motion compensation; Movement; multiphoton; Optical Coherence Tomography; Product design; Radiation; Skin; Skin - diagnostic imaging; Therapeutic applications; Tomography; Tomography, Optical Coherence; Tracking
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.201700131

This paper reports a handheld multiphoton fluorescence microscope designed for clinical imaging that incorporates axial motion compensation and lateral image stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Our system is able to correct lateral sample velocities of up to approximately 65 μm s−1. Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications. The system is used to compensate for both lateral and axial motion of the sample when imaging human skin in vivo. This paper reports the development of a handheld multiphoton microscope with both lateral and axial motion compensation. The system is applied to in vivo imaging of human skin of the dorsal forearm and the chest.