Electropermeabilization by uni- or bipolar nanosecond electric pulses: The impact of extracellular conductivity

Cellular effects caused by nanosecond electric pulses (nsEP) can be reduced by an electric field reversal, a phenomenon known as bipolar cancellation. The reason for this cancellation effect remains unknown. We hypothesized that assisted membrane discharge is the mechanism for bipolar cancellation....

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2018-02, Vol.119, p.10-19
Main Authors: Gianulis, Elena C., Casciola, Maura, Xiao, Shu, Pakhomova, Olga N., Pakhomov, Andrei G.
Format: Article
Language:English
Subjects:
Animals
Bipolar cancellation
Cancellation
Cellular biology
CHO Cells
Conductivity
Cricetinae
Cricetulus
Electric Conductivity
Electric fields
Electric pulses
Electrical resistivity
Electropermeabilization
Electroporation
Electroporation - methods
Extracellular conductivity
Extracellular Space - metabolism
Intervals
Nanosecond electric pulse
Permeability
Polarity
Studies
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Summary:Cellular effects caused by nanosecond electric pulses (nsEP) can be reduced by an electric field reversal, a phenomenon known as bipolar cancellation. The reason for this cancellation effect remains unknown. We hypothesized that assisted membrane discharge is the mechanism for bipolar cancellation. CHO-K1 cells bathed in high (16.1mS/cm; HCS) or low (1.8mS/cm; LCS) conductivity solutions were exposed to either one unipolar (300-ns) or two opposite polarity (300+300-ns; bipolar) nsEP (4–40kV/cm) with increasing interpulse intervals (0.1–50μs). Time-lapse YO-PRO-1 (YP) uptake revealed enhanced membrane permeabilization in LCS compared to HCS at all tested voltages. The time-dependence of bipolar cancellation was similar in both solutions, using either identical (22kV/cm) or isoeffective nsEP treatments (12 and 32kV/cm for LCS and HCS, respectively). However, cancellation was significantly stronger in LCS when the bipolar nsEP had no, or very short (
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2017.08.005