Proportion of N-Type Calcium Current Activated by Action Potential Stimuli

• Published in: Journal of neurophysiology 2005-12, Vol.94 (6), p.3762-3770
• Main Authors: King, J. Darwin, Jr, Meriney, Stephen D
• Format: Article
• Language: English
• Subjects: Action Potentials - physiology; Animals; Anura; Calcium Channel Blockers - pharmacology; Calcium Channels, L-Type - physiology; Calcium Channels, N-Type - physiology; Cells, Cultured; Chick Embryo; Dose-Response Relationship, Radiation; Electric Stimulation - methods; Ganglia, Parasympathetic - cytology; Neurons - drug effects; Neurons - physiology; Neurons - radiation effects; Patch-Clamp Techniques - methods; Temperature
• ISSN: 0022-3077; 1522-1598
• DOI: 10.1152/jn.01289.2004

Department of Neuroscience, University of Pittsburgh, Pittsburgh, Pennsylvania Submitted 16 December 2004; accepted in final form 10 August 2005 N-type calcium currents are important in many neuronal functions, including cellular signaling, regulation of gene expression, and triggering of neurotransmitter release. Often the control of these diverse cellular functions is governed by the spatial and temporal patterns of calcium entry in subcellular compartments. Underlying this issue is the effectiveness of action potentials at triggering calcium channel opening. Chick ciliary ganglion neurons were used as model cells to study the activation of N-type calcium current during action potential depolarization. Several different action potential shapes were recorded, used as voltage command templates, and altered such that control action potential–evoked currents could be compared with those elicited by broadened action potential commands. Depending on the action potential shape used to activate calcium currents in chick ciliary ganglion neurons, and the temperature at which recordings were performed, varying proportions ( I / I max ) of N-type calcium current could be activated. The largest proportion measured occurred using a broad ciliary ganglion cell soma action potential to activate calcium current at 37°C (100%). The smallest proportion measured occurred using a fast, high-temperature–adjusted frog motoneuron nerve terminal action potential to activate calcium current at room temperature (10%). These data are discussed with respect to the impact on cellular signaling and the regulation of transmitter release. Address for reprint requests and other correspondence: S. D. Meriney, Department of Neuroscience, University of Pittsburgh, 446 Crawford Hall, Pittsburgh, PA 15260 (E-mail: meriney{at}bns.pitt.edu )