Substrate stiffness regulated migration and angiogenesis potential of A549 cells and HUVECs

Tumor tissue tends to stiffen during solid tumor progression. Substrate stiffness is known to alter cell behaviors, such as proliferation and migration, during which angiogenesis is requisite. Mono‐ and co‐culture systems of lung cancer cell line A549 and human umbilical vein endothelial cells (HUVE...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular physiology 2018-04, Vol.233 (4), p.3407-3417
Main Authors: Zhao, Dan, Xue, Changyue, Li, Qianshun, Liu, Mengting, Ma, Wenjuan, Zhou, Tengfei, Lin, Yunfeng
Format: Article
Language:English
Subjects:
A549 Cells - metabolism
Angiogenesis
Cancer
Cell culture
Cell Line, Tumor
Cell Movement - physiology
Cell Proliferation - physiology
Endothelial cells
Growth factors
Human Umbilical Vein Endothelial Cells - metabolism
Humans
Lung cancer
Matrix metalloproteinase
Matrix metalloproteinases
Matrix Metalloproteinases - metabolism
Metalloproteinase
migration
Neovascularization, Pathologic - metabolism
Polydimethylsiloxane
proliferation
Silicone resins
Solid tumors
Stiffness
Substrate inhibition
Substrates
tumor
Tumors
Umbilical vein
Up-Regulation
Vascular Stiffness - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tumor tissue tends to stiffen during solid tumor progression. Substrate stiffness is known to alter cell behaviors, such as proliferation and migration, during which angiogenesis is requisite. Mono‐ and co‐culture systems of lung cancer cell line A549 and human umbilical vein endothelial cells (HUVECs), on polydimethylsiloxane substrates (PDMS) with varying stiffness, were used for investigating the effects of substrate stiffness on the migration and angiogenesis of lung cancer. The expressions of matrix metalloproteinases (MMPs) and angiogenesis‐related growth factors were up‐regulated with the increase of substrate stiffness, whereas that of tissue inhibitor of matrix metalloproteinase (TIMPs) were down‐regulated with increasing substrate stiffness. Our data not only suggested that stiff substrate may promote the migration and angiogenesis capacities of lung cancer, but also suggested that therapeutically targeting lung tumor stiffness or response of ECs to lung tumor stiffness may help reduce migration and angiogenesis of lung tumor. Surface morphology of PDMS substrates and the distinctive morphological features of A549 cells cultured on PDMS substrates with varying stiffness.
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.26189