A concordance study of the Altona RealStar Varicella‐Zoster virus real‐time quantitative PCR and in‐house conventional qualitative PCR

This study compared the Altona RealStar™ VZV Kit 1.0 real time quantitative VZV PCR with in‐house qualitative conventional VZV PCR on cerebrospinal fluid, mucocutaneous, and other uncommon clinical specimens. Overall, positive and negative agreement percentages were respectively 97.9% (95%CI: 93.8‐9...

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Bibliographic Details
Published in:Journal of medical virology 2018-02, Vol.90 (2), p.334-337
Main Authors: Grandjean Lapierre, Simon, Boissinot, Karel, Renaud, Christian
Format: Article
Language:English
Subjects:
Assaying
Cerebrospinal fluid
Herpesvirus 3, Human - isolation & purification
Humans
Molecular Diagnostic Techniques - methods
nervous system
Polymerase chain reaction
Polymerase Chain Reaction - methods
Prospective Studies
Real time
skin
Varicella
Varicella Zoster Virus Infection - diagnosis
Varicella‐Zoster virus
Virology
Viruses
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Summary:This study compared the Altona RealStar™ VZV Kit 1.0 real time quantitative VZV PCR with in‐house qualitative conventional VZV PCR on cerebrospinal fluid, mucocutaneous, and other uncommon clinical specimens. Overall, positive and negative agreement percentages were respectively 97.9% (95%CI: 93.8‐99.6), 100.0% (95%CI: 93.1‐100.0), and 96.3% (95%CI: 89.4‐99.2) while Cohen's kappa statistic value was 0.96 (95%CI: 0.91‐1.00). RealStar™ VZV quantitative PCR assay reported average quantitative viral loads of 4.4 × 105 and 1.1 × 107 copies/mL in cerebrospinal fluid and cutaneous specimens, respectively (P 
ISSN:0146-6615
1096-9071
DOI:10.1002/jmv.24957