Genetic, phenotypic and pathogenic diversity among xanthomonads isolated from pistachio (Pistacia vera) in Australia

Repetitive extragenic palindromic polymerase chain reaction (rep-PCR), sequencing of the 16S-23S rDNA internal transcribed spacer (ITS), biochemical and physiological tests, the Biolog microplate system, polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins, and pathogenicity tests were u...

Full description

Saved in:
Bibliographic Details
Published in:Plant pathology 2006-10, Vol.55 (5), p.639-649
Main Authors: Marefat, A, Scott, E.S, Ophel-Keller, K, Sedgley, M
Format: Article
Language:English
Subjects:
16S−23S rDNA sequencing
bacterial diseases of plants
Bacterial plant pathogens
Biolog microplate
Biological and medical sciences
dieback
Fundamental and applied biological sciences. Psychology
genetic variation
geographical variation
host plants
internal transcribed spacers
microbial genetics
molecular sequence data
pathogenicity
pathovars
phenotypic variation
Phytopathology. Animal pests. Plant and forest protection
pistachios
Pistacia
Pistacia vera
polymerase chain reaction
repetitive PCR
ribosomal DNA
SDS–PAGE
strain differences
strains
Xanthomonas
Xanthomonas translucens
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Repetitive extragenic palindromic polymerase chain reaction (rep-PCR), sequencing of the 16S-23S rDNA internal transcribed spacer (ITS), biochemical and physiological tests, the Biolog microplate system, polyacrylamide gel electrophoresis (PAGE) of whole-cell proteins, and pathogenicity tests were used to characterize variability among xanthomonads isolated from pistachio trees suffering from bacterial dieback in four regions of Australia. ITS sequencing and rep-PCR revealed two distinct genotypes among the strains. The ITS sequencing suggested that the pistachio strains were closely related to Xanthomonas translucens pathovars, in particular X. translucens pv. poae. Results of physiological and biochemical tests, as well as Biolog microplate analysis and protein profiling, confirmed the existence of two groups. Furthermore, pathogenicity and host-range studies indicated that the two groups were biologically different. There was an association between the two groups and the geographical origin of the strains.
ISSN:0032-0862
1365-3059
DOI:10.1111/j.1365-3059.2006.01437.x